Ashmarina, M. V.

Chaydonova, V. S.

Tchaikovskaya, Olga N.

English

Ashmarina, Maria V.

Tomsk State University Repository

PULSED LASERS AND LASER APPLICATIONSTHE 15th  INTERNATIONAL CONFERENCEThe 15th International Conference AMPL-2021 PULSED LASERS AND LASER APPLICATIONS September 12–17, 2021 Tomsk, Russia ABSTRACTS GENERAL SPONSOR Special Systems. Photonics, St. Petersburg, Russia CONFERENCE ORGANIZERS Institute of Atmospheric Optics SB RAS Institute of High Current Electronics SB RAS Tomsk State University Tomsk Polytechnic University CONFERENCE SPONSORS Ministry of Education and Science of Russian Federation, Russia Russian Academy of Sciences, Russia Siberian Branch of Russian Academy of Science, Russia Laser Association, Russia  CONFERENCE SPONSORS TOPAZ Research and Inculcation Enterprise, Tomsk, Russia Young Scientists Counsil IAO SB RAS, Tomsk, Russia SP Equipment, Novosibirsk, Russia Azimut Photonics, Moscow, Russia LOTIS TII, Minsk, Belarus Special Systems. Photonics, St.-Petersburg, Russia CLZ Ltd, Moscow, Russia Leningrad Laser Systems, St.-Petersburg, Russia MEDIA SPONSORS Atmospheric and Oceanic Optics Journal, Tomsk, Russia Photonics Journal, Moscow, Russia  Tomsk, 2021  120  BIOPHOTONIS SESSION G  CG-6 INVESTIGATION OF PARAFFIN-EMBEDDED MELANOMA TISSUES  BY MULTIPHOTON MICROSCOPY A.I. Knyazkova1,2, A.A. Samarinova1, L.V. Spirina3,4, A.V. Borisov1,3, and Yu. V. Kistenev1 1Tomsk State University, 36 Lenin Ave., 634050, Tomsk, Russia, yuk@iao.ru; 2Institute of Atmospheric Optics SB RAS, 1 Zuev Sq., 634055, Tomsk, Russia; 3Siberian State Medical University, 2 Moskovsky Trakt St., 634050, Tomsk, Russia; 4Cancer Research Institute of Tomsk NRMC, 5 Kooperativny Lane, 634009, Tomsk, Russia, SpirinaLV@oncology.tomsk.ru The traditional diagnosis of skin cancer is by biopsy and histopathological examination. Among newly developed imaging techniques, two photon microscopy is one of the promising method for diagnosing pathological tissue changes at oncological diseases. Herein, we demonstrate the feasibility of using multiphoton microscopy combined with FLIM to detect morphological and metabolic state changes tissues whith melanoma, embedded in paraffin blocks. The method is based on imaging the amplitude ratio 1∕2, attributed to the ratio of the free and protein bound forms of NADH, as a metabolic marker. The relationships between the fluorescence and the generation of the second harmonic of the melanoma tissues and quantitative parameters of fluorescence lifetimes (m, 1, 2, and 1∕2) were obtained for different morphological images.  This work was performed within the frame partial financial support from the Russian Foundation for Basic Research (Grant No. 20-32-90098) and supported by the Government of the Russian Federation (proposal No. 2020-220-08-2389 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions, Russian institutions of higher education) G-7 METHODS FOR DETERMINING THE RESIDUAL ANTIBIOTICS IN FOOD O.N. Tchaikovskaya1, V.S. Chaydonova1,2 and M.V. Ashmarina1 1Tomsk State University, 36 Lenin Ave., 634050, Tomsk, Russia, tchon@phys.tsu.ru; 2Hygienic and Epidemiological Center in Republic of Khakassia, 66 Lenin Ave., 655017, Abakan, Russia, krayvlada0523@mail.ru Now one of the pressing issues for the sanitary service is the problem of determining antibiotics in livestock products. Because their use in animal agriculture has more and more diverse purposes. First of all for the treatment of animals, as the cheapest and effective way. However, in addition, they are used to stimulate growth, preserve and package food (meat, fish, milk, honey, etc.). Because of what they can enter the body with food, which harms not only pathogenic bacteria, but also the beneficial microflora of the body. Also, their constant presence in the human body can lead to antibiotic resistance and are the strongest allergen. Among other things, their inclusion can provoke the development of various diseases. It is now important to find a sensitive and accurate method for the determination of antibiotics [1, 2] in livestock products, which would exclude lengthy sample preparation. Therefore, the development of a fast method for the determination of antibiotics in food is fast and urgent. The spectral method based on the phenomenon of fluorescence can be a solution to this problem. Acknowledgments: The reported study was funded by RFBR, Project No. 20-32-90116. AOAC Official Methods of Analysis. Chlortetracycline, Oxytetracycline, and Tetracycline in Edible Animal Tissues. Liquid Chromatographic Method, Drugs and Feed Additives in Animal Tissues, 2000, Ch. 2, 20.  1. Bautista J.A.G., Mateo J.V.G. and Calatayud J.M. // Anal. Lett. 1998. V. 31. P. 1209–1218. 

Methods for determining the residual antibiotics in food

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Methods for determining the residual antibiotics in food

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