DNA Isolation from Desiccated Leaf Material for Plum Tree (Prunus domestica L.) Molecular Analysis

Abstract

Conservation of biodiversity is very important, especially in species such as plum tree. One of the key aspects is the establishment of appropriate genetic markers that could be used in identifying provenances and also for breeding purposes. In this context, molecular characterization using SSR (short sequence repeats) markers is very useful and was chosen for our study. This article describes the assessment of a CTAB-based DNA extraction protocol for isolating and purifying DNA from the plum cultivars selected for molecular characterisation. The quality of DNA extracts was assessed by spectrophotometric measurement and their suitability for molecular analysis was evaluated using a SSR marker system. We concluded that the DNA extraction protocol is suitable for obtaining plum DNA that can be used for molecular analysis using SSR markers

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