This paper deals with the effect of soil particle size on copper availability. Twelve vineyards, (at three depths) all on the territory of the Vojvodina Province, were observed for soil contamination with copper. Soil samples were taken in four locations (5 soil types). The samples were analyzed for total copper and available copper (in EDTA). and sequential extraction was conducted. Correlations between soil particle size and copper fractions were calculated and analyzed. The obtained results indicated that the increase in the portion of smallest soil separates, clay and silt, tended to reduce copper availability, whereas the larger separates tended to increase it. The available fractions, C u e x and C u c a r , were significantly negatively correlated with the content of clay and silt along the entire soil profile of the analyzed vineyards. Simultaneously, these two factions were positively correlated with the content of fine sand, also along the entire soil profile. The results confirmed that, when assessing the extent of soil contamination with copper, soil mechanical composition should be taken into account as an important factor of copper availability