Hartung, Susen

Kreuzig, Robert

Smalla, Kornelia

Widyasari, Arum

Wolters, Birgit

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2013 Wolters et al.     Detection of antibiotic resistance genes and mobile genetic elements in piggery manures and fermentation residues from biogas plants  Birgit Wolters1, Susen Hartung2 , Arum Widyasari2 , Robert Kreuzig2 and Kornelia Smalla1 1Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig 2Technical University of Braunschweig, Institute of Environmental and Sustainable Chemistry, Braunschweig Email of corresponding author: birgit.wolters@jki.bund.de  Antibiotics are used extensively in ani-mal husbandry and treated animals often excrete them unchanged or as metabolites. These antibiotics lead to a fitness advantage of resistant bacteria present in the intestine of the animals and in agro-ecosystems to which ma-nures and fermentation residues are applied as fertilizers. This might lead to an enrichment of resistant bacteria and corresponding antibiotic resistance genes (ARGs) in the microbial commu-nities. If they are located on mobile genetic elements (MGEs) these genes may spread even among phylogenetically distant bacteria and new resistance phenotypes of patho-gens might occur.  The aim of our project is to monitor antibiotics and ARGs in manures and fermentation residues in order to de-velop mitigation strategies and to min-imize the risk of resistance transfer to pathogens.  To identify parameters influencing the degradation of antibiotics and reduc-tion of ARGs and MGEs during fermen-tation in biogas plants or storage of manure we sampled 8 mesophilic bio-gas plants (fermenters were fed with pig manure except one which was fed with bovine manure) at different pro-cessing steps and stored manures of 16 different pig producing facilities. The samples were analyzed for the abun-dance of ARGs and MGEs (via PCR, Southern blot and quantitative real time PCR) as well as for the presence and content of antibiotics (via LC/MS/MS).   By using DGGE of 16S rRNA gene frag-ments amplified from total community DNA we analyzed differences in the composition of bacterial communities in manure from different farms and during fermentation in biogas plants.  We detected plasmids of different in-compatibility groups (IncN, IncQ, IncP-1, IncW, IncU, Low GC), integase genes of class 1 and class 2 integrons, several ARGs (sul1, sul2, sul3, tetA, tetM, tetX, aadA1) and the resistance gene qacE∆1 (against quaternary ammonium com-pounds) in many samples.   Our data indicate that piggery manures as well as fermentation residues from biogas plants might contribute to the spread of ARGs and MGEs in the agro-ecosystem when used as fertilizer. Berichte aus dem Julius Kühn-Institut 171 47

Detection of antibiotic resistance genes and mobile genetic elements in piggery manures and fermentation residues from biogas plants

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Detection of antibiotic resistance genes and mobile genetic elements in piggery manures and fermentation residues from biogas plants

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