Expression of avian cellular transcripts in FFs associated to their growing feathers at microdissection and after <i>in vitro</i> cultivation.

Abstract

RNAs were extracted from FFs with a shortened feather after microdissection (D0), and after 3, 5, and 7 days of culture. Following cDNA synthesis, real-time qPCR was performed. At each time point, data from three samples (of 2 FF each), from 2 independent experiments, with qPCR technical repeats are shown. A. RNA levels of each gene at Day 0 were calculated relatively to the housekeeping gene RPS17 from freshly microdissected FF expressed in arbitrary units (A.U) and presented in the bar graph with median and interquartile range. B. RNA expression of the various markers was normalized to the housekeeping gene RPS17, and the fold-changes at Day 3, 5, and 7 relative to Day 0 are shown in a dot plot with median and interquartile range. No significant difference in the expression of NCAM, LCAM, Wnt6 and BMP4 was observed at any time point. In contrast, the expression of KRT14, HBS-1, Notch1, DKK3 and Shh changed significantly at several time points during the culture. Significance of differences was performed relatively to Day 0 by using the Kruskal-Wallis test with a Dunn correction for multiple comparison (adjusted p-value >0.5, ns; p-value <0.5, *; p-value<0.01, **; p-value <0.0001, ****).</p