淹水、高盐和土壤缺氧等不良环境因子导致了红树植物组织富含多酚、色素、黏多糖等次生代谢物,激素测定有一定困难.本文以胎生红树植物——木榄(Bruguiera gymnorrhiza(L.)Lamk)为研究对象,探讨红树植物内源激素——脱落酸(ABA)和赤霉素(GA3)的提取纯化方法以及合适的HPLC色谱测定条件.结果表明:采用等度洗脱、35%甲醇(含0.15%0.1 mol/L H3PO4)流动相、流速1.0 mL/min,分别在245和208 nm下检测,ABA和GA3分离效果理想,回收率分别达到99.8%和95.5%.整个过程简单、准确、易操作.比较木榄的花蕾、种子、幼胚轴、成胚轴中ABA和GA3含量变化,种子和幼胚轴ABA含量较低,而GA3含量较高,这可能是影响红树植物胎生现象发生的重要原因之一.Mangrove plant produces high levels of secondary metabolites(polyphenolics,pigment and mucopolysaccharide,etc.) in response to environmental factors such as hypersaline stress,waterlogging and low oxygen level in sediment,which hinder the extraction of hormones.In this paper,the extraction and purification methods and the operation condition of reversed-phase high performance liquid chromatography(RP-HPLC) were studied for a quantitative analysis of abscisic acid(ABA) and gibberellin acid(GA3) in Bruguiera gymnorrhiza Lamk,a viviparous mangrove species.Under the conditions of isocratic concentration 35% methanol with 0.15% 0.1 mol/L H3PO4,flow rate 1.0 mL/min,UV wavelength 245 nm for ABA and 208 nm for GA3,two mangrove plant hormones recoveries were high up to 95.5% and 98.8% respectively.This HPLC quantitative analysis method is convenient,fast and accurate.Compared with flower buds and mature hypocotyles,seeds and young hypocotyles had lower level of ABA and higher level of GA3,which might contribute to the viviparity of mangrove plant.福建省自然科学基金(B0410001)资助项
