Overexpression of <i>RECQL4</i> results in increased RAD51 foci and decreased tail moment.

Abstract

(A) Overexpression of RECQL4 results in increased RAD51 foci, which is dependent on its helicase activity. U2OS cells were transfected with an empty plasmid or a plasmid expressing RECQL4 or RECQL4-K508A under a CMV promoter. The cells were either mock or cisplatin treated for one hour and after a two-hour recovery, imaged for RAD51 foci or DAPI by immunofluorescence. RAD51 foci was quantified from 200 cells per condition for each experiment. The experiment was performed three to five times and the median was graphed (Unprocessed foci count in S9 Data). Representative images are shown. (B) Overexpression of RECQL4 results decreased tail moment following cisplatin exposure, which is dependent on its helicase activity. U2OS cells were treated similarly to the immunofluorescence experiment, before being harvested for neutral comet assay. At least 40 comets were counted per condition for each experiment. The experiment was performed four times and the mean and standard deviation was graphed (Unprocessed tail moments in S10 Data).</p