9-Myc tagging Hrq1 does not result in cisplatin sensitivity.

Abstract

(A) Hrq1-9myc cells displayed similar cisplatin sensitivity compared to WT cells. The indicated yeast strains were five-fold serial diluted onto SC medium containing DMSO and/or SC medium containing the indicated amount of cisplatin. The plates were photographed after 2 days of incubation at 30°C in the dark. (B) Hrq1 protein level is unchanged with HU and MMS treatment. Hrq1-9myc cells were grown to log phase and then treated with indicated agents for two hours before being collected for TCA prep. Cisplatin was used at 100 μg/ml (Raw densitometry data in S11 Data). (TIF)</p