Spatial patterns of sharka disease, caused by plum pox virus (PPV) and vectored by several species of aphid, were determined by double antibody sandwich-enzyme-linked immunosorbent assay using polyclonal antibodies in newly infected, mature apricot and peach orchards in eastern Spain. Among yearly assessments of plots examined for within- and across-row aggregation of adjacent sharka-diseased trees, only a few transects were found to have aggregation by ordinary runs analyses. Analyses, using beta-binomial index of dispersion (I-beta) to determine if spatial aggregation was present in each plot for data partitioned into quadrats of different spatial dimension, demonstrated occasional aggregation and results were generally inconclusive. Significant (I-beta) values, when present, were generally found associated with plots with higher disease incidence. No disease gradients were discernible for any of the plots and years. More rigorous spatial analyses were used to test for spatial relationships over longer distances. Two-dimensional distance class analyses indicated a spatial dependency of PPV-infected stone-fruit trees over distance, a general scarcity of significant distance classes near the origin, and the presence of significant distance classes occasionally comprising small loose clusters at distances near the center or distal end of the proximity matrices especially during the initial stages of the epidemics. Geostatistical analysis confirmed the lack of significant associations among immediately adjacent trees and the trend for higher order spatial associations in semivariograms for distances corresponding to the center and distal ends of the proximity matrices. This trend in semivariance over distance was best described by linear or exponential increase models compared with transitional models commonly used in geostatistics. Correlation analysis indicated a significant conservation of orientation of localized systemic infections in scaffold branches over years. The spatial patterns of sharka suggest the lack of movement of PPV-viruliferous aphid vectors to immediately adjacent trees and their preferential movement to trees several tree spaces away
