Characteristicsof Chemical Compound Content in Meniran Herb Extract and Miana Leave Extract Based On Phytochemical Screening and Thin Layer Chromatography
Meniran herbs and miana leaves have been scientifically proven as immunostimulant, antibacterial and antituberculosis. The pharmacological activity of herbs is determined by the content of chemical compounds. The purpose of identifying chemical compounds in meniran herb extract and miana leaf extract based on phytochemical screening and thin layer chromatography (TLC). The extraction method is by maceration using 96% ethanol as solvent. Phytochemical screening includes alkaloids, tannins, saponins, flavonoids, phenols, glycosides, steroids, terpenoids, phlobatins and anthraquinones. TLC identification uses 4 eluent compositions, namely: Chloroform: Methanol: Water = 15:6:1; Ethyl acetate : ethanol : water = 16:5:1; Benzene : ethyl acetate = 7:3 and N hexane : ethyl acetate = 8:2. Phytochemical screening results showed that Meniran herb extract contained alkaloids, tannins, saponins, flavonoids, phenols, steroids and terpenoids. Miana leaf extract contains alkaloids, tannins, saponins, flavonoids (flavones), phenols, steroids, terpenoids, phlobotanins and anthraquinones. The results of thin layer chromatography showed that the meniran herb extract identified 1 polar compound, 17 semi-polar compounds and 7 non-polar compounds. Miana leaf extract identified 1 polar compound, 21 semi-polar compounds and 17 non-polar compounds
