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Magnetic resonance microscopy in cardiac development
Authors
Agard
Andersen
+60 more
Ayoob
Baird
Brink
Chicurel
Dabiri
Doyle
Dunn
Eckert
Elfgang
Falk
Falk
Falk
Falk
Falk
Falk
Falk
Friend
Fujimoto
Gilula
Ginzberg
Gross
He
Heikal
Heim
Heim
Holm
Hülser
Inouye
Jiang
Jordan
Keller
Kumar
Laird
Li
Makowski
Matz
McAdara
McNutt
Miesenböck
Milks
Misteli
Miyawaki
Murray
Musil
Ormö
Prasher
Rash
Revel
Risek
Rizutto
Shah
Shaw
Stauffer
Sullivan
Terskikh
Tsien
Unger
Wall
Yang
Yeager
Publication date
1 January 2001
Publisher
'Wiley'
Doi
Abstract
Magnetic resonance microscopy (MRM) is a fast and non-destructive imaging technique that can analyze the three-dimensional structure of the embryonic heart both qualitatively and quantitatively. Intravascular contrast agents have been developed to accentuate the anatomy of cardiac chambers, the cardiac outflow tract, and major arteries and veins throughout the embryonic body. MRM generates non-distorted three-dimensional data of vascular anatomy in a fraction of the time required by conventional optical image reconstruction techniques. The three-dimensional nature of these data allows the creation of visual models that can be manipulated for fast and easy interpretation of the complex relationships between heart chambers and aortic arches. This is particularly helpful because these relationships change in complex ways during development. The non-destructive nature of MRM makes it well suited for investigating rare or valuable specimens and live subjects. MRM techniques have been developed for imaging the embryo in utero and in vitro, although MRM studies of fixed embryo specimens are easier to perform and produce data with better contrast and higher resolution. Microsc. Res. Tech. 52:323–330, 2001. © 2001 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35183/1/1016_ftp.pd
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