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Previous issue date: 26O presente estudo objetivou analisar a captura, transporte, adaptação e reprodução de matrizes selvagens de Lophiosilurus alexandri às condições de laboratório. Foram realizadas duas campanhas para coletas de exemplares a partir de habitats naturais. Os animais sobreviventes da primeira (7) e segunda (17) captura foram divididos em quatro tanques de 5 m3, sendo em dois deles adicionada camada de areia e mantidos a temperatura de 28ºC. As desovas ocorreram de forma natural 30 dias após o aumento da temperatura, mas somente nos tanques com fundo de areia. No primeiro período reprodutivo foram coletados 24 desovas nestes tanques. No segundo período reprodutivo os animais foram sexados por canulação e distribuídos novamente em quatro tanques, revestidos com areia no fundo e mantidos a temperatura de 28ºC. Em todos os tanques ocorreram desovas num total de 36. Durante a etapa de reprodução, foram coletadas amostras de ovos, larvas recém eclodidas (LRE) e larvas no final do período lecitotrófico (oito dias pós eclosão) (LFL) para determinação do perfil de aminoácidos e de ácidos graxos. A proteína bruta não apresentou variações no decorrer do desenvolvimento (P > 0,05) e a concentração de lipídeos foi superior em LRE (P 0.05) and concentration of lipids was highest in NHL (P < 0.05). Glutamine was the amino acid (AA) found in the highest concentrations in eggs, NHL, and LPL. The essential amino acids (EAA) isoleucine, leucine, and valine decreased in LPL, while in eggs and NHL, the values were higher and similar to each other. Arginine and lysine showed lower and similar amounts in eggs and NHL and increased in LPL. The dispensable amino acids (DAA), such as aspartic acid,tyrosine, and glycine increased in LPL while alanine decreased in this stage of life. The neutral lipids (NL) percentage increased in LPL larvae. The SFA (saturated fatty acids) decreased during ontogeny, while MUFA (monounsaturated fatty acids) decreased only in LPL. The PUFA (polyunsaturated fatty acids) was highest in LPL, probably due to increased percentages of the fatty acids Arachidonic (20:4n-6) (ARA), Eicosapentaenoic (C20:5n3) (EPA), and Docosahexaenoic (C22:6 n3) (DHA). Polar fatty acids (PL) were found in larger percentages in eggs and NHL and decreased in LPL. The SFA decreased during ontogeneticdevelopment, while MUFA decreased only in LPL. The PUFA was highest in LPL, probably due to the increased percentage of ARA and DHA. The percentage of EPA remained unchanged throughout the experiment. The pacamã is able to adapt and reproduce inlaboratory conditions, and it is possible to manipulate its reproductive period by controlling the water temperature and food. Protein is kept during ontogenetic development although there were changes in the amino acid classes, such as a decrease (IAA) of isoleucine, leucine,and valine in LPL. L. alexandri preferably use SAFAs and MUFAs as an energy source, from both the NL and PL fractions, during its early development
