Abbas Lubna

Bakacsy László

Szekeres András

Vigneshwari Aruna

Vágvölgyi Csaba

Škrbić Biljana

English

University of Szeged

 Book of Abstracts | 16th Wellmann International Scientific Conference| ISBN 978-963-306-589-1  95  ANTIMICROBIAL ACTIVITIES OF THE SECONDARY METABOLITES OF ENDOPHYTIC FUNGI ISOLATED FROM JUNIPERUS COMMUNIS  ARUNA VIGNESHWARI1, LUBNA ABBAS1, LÁSZLÓ BAKACSY2, BILJANA ŠKRBIĆ3, CSABA VÁGVÖLGYI1, ANDRÁS SZEKERES1  1Department of Microbiology, University of Szeged,  Közép fasor 52, H-6701, Szeged, Hungary 2Department of Plant Biology, University of Szeged Közép fasor 52, H-6701, Szeged, Hungary; 3Faculty of Technology, University of Novi Sad Bulevar cara Lazara 1., 21000 Novi Sad, Serbia  szandras@bio.u-szeged.hu (A.S.)   The microbial endophytes are important components of the plant micro ecosystem residing in healthy internal tissues of plants asymptomatically. These microorganisms play a significant role in influencing the synthesis of metabolic products in plants and they are proved to be an excellent reservoir of bioactive compounds.  The aim of the present study was to isolate the endophytic fungi from the medicinal plant Juniperus communis and evaluating the antimicrobial potential of these isolates. Altogether, 138 endophytic fungi were isolated from 217 cuttings of healthy twigs, roots, cones and leaves of J. communis. Out of 138 isolates, 80 strains were cultivated in shaken flask cultures; after the cultivation both the mycelia and ferment broth were extracted with the mixture of chloroform and methanol (4:1). The crude extracts were tested for their antimicrobial potential against six bacteria such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptomyces aureus and Micrococcus luteus. Amongst 160 extracts, 78 extracts showed more than 90% inhibitory activity against a minimum of one tested bacterium. Most of them were active against B. subtilis (77), Staph. aureus (93), Strept. aureus (101) and P. aeruginosa (65) showing inhibition over 60%, whereas E. coli and M. luteus were barely inhibited. The extracts were also tested against two fungal species: Candida albicans and Aspergillus niger. Three ferment broth extracts of strains isolated from twigs of the host plant caused remarkable zone of inhibition against both fungi. The mycelial and ferment broth extract of strain J.S 29 exhibited the highest inhibition zone (~50 mm after 3 days) against C. albicans.  Our results highlighted that J. communis harbours many endophytic fungi, which have remarkable potential to produce bioactive compounds.  Book of Abstracts | 16th Wellmann International Scientific Conference| ISBN 978-963-306-589-1  96  Acknowledgements This work was supported by the Hungarian Government and the European Union within the frames of the Széchenyi 2020 Programme through grant GINOP-2.3.2-15-2016-00012. AS was supported through the New National Excellence Program of the Ministry of Human Capacities (ÚNKP-16-4). The infrastructural background was established with the support of GINOP-2.3.3-15-2016-00006 grant (Széchenyi 2020 Programme). BS participation was supported through the project TÉT_16-1-2016-0148 (National Research, Development and Innovation Fund of Hungary). 

Antimicrobial activities of the secondary metabolites of endophytic fungi isolated from Juniperus communis

http://acta.bibl.u-szeged.hu/73034/1/wellmann_abstracts_2018_095-096.pdf

Antimicrobial activities of the secondary metabolites of endophytic fungi isolated from Juniperus communis

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