Buskin A

Crawford SE

Curry EL

Franco OE

Gaughan L

Hayward SW

Heer R

Hepburn AC

Mills IG

Moad M

Robson CN

Singh P

Steele RE

Wilson L

\ua9 2020 The Authors. STEM CELLS TRANSLATIONAL MEDICINE published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complimentary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease

Newcastle University E-Prints

Propagation of human prostate tissue from induced pluripotent stem cells

Propagation of human prostate tissue from induced pluripotent stem cells

Abstract

Similar works

Full text

Available Versions

Newcastle University E-Prints