Not AvailableMolecular identity of dominant cultivars of
soybean needs to complement the morphological descriptors, which are insufficient and influenced by environment,
for protecting the interest of breeders and maintaining the
genetic purity. In the present investigation, genomic DNA
of six commercial Indian soybean varieties viz. JS95-60,
JS93-05, NRC7, JS97-52, NRC37, JS335 was amplified
using ten simple sequence repeat (SSR) markers, which
were selected based upon the relatively higher polymorphic
information content (PIC) value observed after polymorphism survey of 46 soybean genotypes through 58 SSR
markers. PCR analysis showed nine polymorphic SSR
primers which could differentiate unambiguously all the
six varieties. Maximum similarity was observed between
NRC7, JS93-05 and JS95-60. Two unique alleles amplified
in each of the two varieties namely JS335 and NRC37 and
four unique alleles in JS97-52 unambiguously differentiated them from the remaining varieties. Morphologically
similar varieties JS93-05 and JS95-60 could be differentiated distinctly by primer pair Satt181, while NRC7 could
be distinguished from both JS93-05 and JS95-60 through
Sct_199. Results also showed that these nine polymorphic
markers could distinguish the dominant cultivars from six
non-trading varieties, which further confirmed the utility of
nine SSR primers for protecting the breeders’ rights and
maintaining the purity of dominant cultivars during seed
production programme.Not Availabl
