Background. Duck treatments and vaccine designs against the opportunistic human pathogen Pseudomonas aeruginosa have multiple issues, all associated with the diverse genetic traits present in this pathogen, ranging from multi-drug resistant genes to the molecular machinery for the biosynthesis of biofilms. Several candidate vaccines against P. aeruginosa have been developed, which target the outer membrane proteins; However, major issues arise when attempting to establish complete protection against this pathogen due to its presumably genotypic variation at the strain level. To shed light on this concern, we propose this study to assess the P. aeruginosa pangenome and its molecular evolution across multiple strains. Results the P. aeruginosa pangenome was estimated to contain almost 17,000 non-redundant genes, and approximately 15% of these constituted the core genome. Functional analyzes of the accessory genome indicated a wide presence of genetic elements directly associated with pathogenicity. An in-depth molecular evolution analysis revealed the full landscape of selection forces acting on the P aeruginosa pangenome, in which purifying selection drives evolution in the genome of this human pathogen. We also detected distinctive selection wide variety of outer membrane proteins, with the data supporting the concept of substantial genetic variation in proteins probably recognized antigens. Approaching the evolutionary information of genes under extremely positive selection, we designed a new multi-locus sequencing typing assay for and informative, rapid and cost-effective genotyping of P. Aeruginosa on a large scale.Departamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colciencias5817-569-34856Establecimiento de módulo funcionales de secuencia en familias de metiltransferasas de RNA y su uso en la búsqueda de genes de resistencia a antibióticos en patógenos humanosn
