A. A. Fouladi-Nashta

Adamia

Allegrucci

Baerwald

Bodevin-Authelet

Camenisch

Campbell

Deroo

El Maradny

Erickson

Fortune

Freudenberger

G. M. Hartshorne

Galluzzo

Hall

Hettinger

Hunter

Itano

Jacob

Jain

Juengel

Koehler

Kosaki

Kultti

Laurent

Manna

Marei

Marzioni

Miyake

Moriarty

Necas

Nykopp

Ohta

Perry

Pettersson

R. Chavoshinejad

Raheem

Rilla

Robinson

Sainio

Saito

Scaramuzzi

Schoenfelder

Silva

Stern

Tempel

Tienthai

Toole

Tsonis

Ulbrich

W. F. A. Marei

Watson

Weigel

Zhao

English

Chavoshinejad, R

Marei, W F A

Hartshorne, G M

Fouladi-Nashta, A A

RVC Research Online

Localisation and endocrine control of hyaluronan synthase (HAS) 2, HAS3 and CD44 expression in sheep granulosa cells

The aim of the present study was to investigate the hormonal regulation of hyaluronan (HA) components in sheep granulosa cells. HA components are present in the reproductive tract and have a range of physical and signalling properties related to reproductive function in several species. First, abattoir-derived ovaries of sheep were used to determine the localisation of HA synthase (HAS) 1–3 and CD44 proteins in antral follicles. Staining for HAS1–3 and CD44 proteins was most intense in the granulosa layer. Accordingly, the expression of HAS2, HAS3 and CD44 mRNA was measured in cultured granulosa cells exposed to 0–50 ng mL–1 of 17β-oestradiol and different combinations of oestradiol, gonadotropins, insulin-like growth factor (IGF)-1 and insulin for 48–96 h (1 ng mL–1 FSH, 10 ng mL–1 insulin, 10 ng mL–1 IGF-1, 40 ng mL–1 E2 and 25 ng mL–1 LH.). mRNA expression was quantified by real-time polymerase chain reaction using a fold induction method. The results revealed that the hormones tested generally stimulated mRNA expression of the genes of interest in cultured granulosa cells. Specifically, oestradiol, when combined with IGF-1, insulin and FSH, stimulated HAS2 mRNA expression. Oestradiol and LH had synergistic effects in increasing HAS3 mRNA expression. In conclusion, we suggest that the hormones studied differentially regulate HAS2, HAS3 and CD44 in ovine granulosa cells in vitro. Further work is needed to address the signalling pathways involved.</jats:p

Crossref

Reproduction Fertility and Development

https://researchonline.rvc.ac.uk/id/eprint/10237/1/10237.pdf

Localisation and endocrine control of hyaluronan synthase (HAS) 2, HAS3 and CD44 expression in sheep granulosa cells

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