Abstract: Bamboo is a fast growing woody grass which is abundon in ponds of enzali port &Majnun Island in the North and South of Iran respectively that has great potential to be used as fuel ethanol production. It contains about 40% cellulose and 27% hemicelluloses respectively. In this investigation,bamboo was pretreated with dilute sulfuric acid prior to enzymatic hydrolysis process. The amount of dry feedstock solid liquid looking at 10% w/w was pretreated in an autoclave at different temperature (140ºC) with different residence times (30,90min.) and different sulfuric acid concentration (0.6, 1.2% w/w). Results showed that maximum glucose and xylose yields were achieved at 140ºC, 1.2% sulfuric acid concentration and 90min. After followed by enzymatic saccharification with cellulose and B-glucosidase at the same pretreatment condition the yields of total reducing sugars were lose