750 research outputs found

    Optimization for Medium and Cell Disruption of Haematococcus pluvialis

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    虾青素是一种重要的类胡萝卜素,具有良好的着色功能、超强的抗氧化活性、抗肿瘤、增强免疫力和延缓衰老的作用,在水产养殖、食品添加剂、医药保健品等方面有着广泛的应用前景。雨生红球藻是天然虾青素最好的来源,虾青素含量可达藻体干重的4%,而且该来源的虾青素最易被动物体吸收,因此,雨生红球藻的研究正受到越来越广泛的关注。 本文首先对实验室两个品系雨生红球藻藻株HaematococcuspluvialisH(简称HPH)和HaematococcuspluvialisM(简称HPM)进行了比较研究,确定了以HPM为本研究的实验材料,然后分别研究三种培养基BBM、BBM+V、3NBBM+V对藻体生长及虾青素积...Astaxanthin, an important carotenoid, has been increasingly used in aquaculture industry, food additives, medicine and health products because of its color feature, excellent antioxidant activity, anti-tumor function. As chemical synthesized astaxanthin exists an application security issue, the demand for natural astaxanthin is growing. Haematococcus pluvialis, as a best natural source of astaxant...学位:理学硕士院系专业:化学化工学院_化学生物学学号:2052011115157

    Study on the effects of genetic transformation of isopentenyl diphosphate isomerase gene on the astaxanthin content of Haematococcus pluvialis

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    虾青素是一种抗氧化活性极强的类胡萝卜素,具有广泛的应用价值。雨生红球藻是自然界虾青素含量最高的生物,能在逆境胁迫条件下大量合成并积累虾青素,其虾青素积累量可高达细胞干重的4%。但生物量提高与虾青素积累的矛盾限制了虾青素的规模化生产,因此通过基因工程手段改造雨生红球藻的生物学特性,提高其生长速度和虾青素含量具有重要意义。 本研究克隆了虾青素合成过程中的关键酶——IPP异构酶基因,利用建立的农杆菌侵染转化体系和基因枪法将ipiHp1基因导入雨生红球藻细胞内,分析过表达IPP异构酶基因的雨生红球藻株生物量及虾青素含量的变化。主要研究成果如下: 1.建立了农杆菌介导的雨生红球藻遗传转化的筛选体系,...Astaxanthin, a kind of red ketocarotenoid, has extensive application value because of its strong antioxidant ability. It is generally accepted that Haematococcus pluvialis can accumulate the highest levels (up to 4% dry weight) of astaxanthin under environmental stress among the natural creatures. But the large-scale production of astaxanthin is limited by the contradiction between biomass increas...学位:理学硕士院系专业:生命科学学院_细胞生物学学号:2162010115243

    Separation and purification of astaxanthin from Phaffia rhodozyma and the immunomechanism of astaxanthin

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    虾青素是一种具有极强抗氧化和抗衰老活性的类胡萝卜素,被誉为“超级维生素E”,在医药、保健和化妆品等方面有着广泛的应用价值。为了满足日益增加的市场需求,急需进一步降低虾青素的生产成本,目前市售虾青素主要来源是雨生红球藻,而对于红发夫酵母来源虾青素的分离提纯却鲜有报道;另一方面,虽然虾青素强大的生理功能已逐渐被人所知,但对其免疫功能及其作用机理尚未有深入的研究报道,为使虾青素在人类健康方面更好地发挥作用,对其免疫机理的研究也是非常必要的。 本文首先对红发夫酵母虾青素的分离纯化进行研究。采用高压匀浆破碎法破碎红发夫酵母细胞,考察不同破碎压力、破碎次数和处理量对细胞破壁及虾青素萃取率的影响;结果表明...Astaxanthin, one kind of high-effective antioxidative and antiaging carotenoid, is well-known as "super vitamin E"and widely utilized in pharmaceuticals, cosmetics and health care fields. In order to meet the increasing market demand, it is very urgent to reduce the cost of astaxanthin production. Haematococcus pluvialis is the main soure of commercial astaxanthin currently. However, no special at...学位:工学硕士院系专业:化学化工学院_化学工程学号:2062010115143

    The molecular mechanism of antioxidant effect of astaxanthin and preparation of its nanosuspensions

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    虾青素(astaxanthin,3,3'-二羟基-4,4'-二酮-β,β'胡萝卜素)属于叶黄素类β-胡萝卜素。研究发现虾青素对人类健康很有益处,对许多疾病都有很好的治疗保护作用。它主要的药理作用包括:抗氧化、抗肿瘤、抗炎、对心血管保护作用及神经保护作用等。 前期研究表明,虾青素的抗溃疡作用与氧化应激密切相关,但未在分子水平进行深入研究。因此,本课题以胃癌细胞为研究对象,采用Westernblot法检测虾青素刺激后氧化还原信号通路关键蛋白的激活情况,通过DCFH-DA法检测细胞内ROS的改变,分析他们之间的相关性。结果显示,ERK5的激活与细胞内ROS的量之间有一定的相关性,我们通过PEI法建...Astaxanthin, 3,3'-dihydroxy-β,β'-carotene-4,4'-dione, belongs to the family of xanthophylls. Astaxanthin has considerably potential and promising applications in human health and nutrition,and has been attributed with extraordinary potential for protecting the organism against a wide range of diseases. Most significant activities of astaxanthin including its antioxidative, anticancer, antidi...学位:理学硕士院系专业:药学院_药理学学号:2452009115302

    Regulation Mechanism of the Carbon and Nitrogen on Astaxanthin Synthesis in Phaffia rhodozyma by Proteomics

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    随着人类对天然虾青素需求的不断扩大,红发夫酵母以其易于培养的优点被选作当前虾青素生产极具潜力的微生物。过去研究发现培养基中氮源对红发夫酵母虾青素合成有明显的影响,低氮能够促进红发夫酵母合成虾青素。因此,本论文研究了碳氮源对红发夫酵母生长及虾青素产量的影响,继而采用蛋白质组学技术研究在碳氮源调控下红发夫酵母细胞表达的差异蛋白,筛选出响应碳氮源的关键蛋白质,并从分子水平上初步探索碳氮源调控机制。 首先,本论文在对碳氮比优化时发现初始葡萄糖浓度对红发夫酵母中虾青素的积累有很大的影响,在氮源浓度一定的条件下,高浓度葡萄糖可促进红发夫酵母生物量的积累及虾青素产量的提高,但虾青素的比产率却较低浓度葡萄糖...With the increasing demand of human beings for natural astaxanthin, Phaffia rhodozyma which was easily cultured was chosen as the most potential production microorganism. The initial nitrogen source remarkably affected astaxanthin biosynthesis in P. rhodozyma. Low nitrogen content also promoted the biosynthesis of astaxanthin of P. rhodozyma. Therefore, this study aimed to reveal the differential...学位:工学硕士院系专业:化学化工学院_生物化工学号:2062010115150

    Studies of cultures medium and cultures modes of Haematococcus pluvialis for enhanced production of astaxanthin

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    虾青素以其卓越的生理功能,在国际上已被广泛应用于水产养殖、食品及医药业中。雨生红球藻被公认为自然界中生产天然虾青素的最佳生物来源,因此利用雨生红球藻获取虾青素,无疑具有广阔的发展前景。本研究建立了一种雨生红球藻中的虾青素酯皂化进行分离测定的反相高效液相色谱方法。虾青素在15min内得到了较好的分离,其中虾青素的检出限为0.049mg/L,在1-15mg/L范围内虾青素的含量与峰面积具有良好的线性关系;该方法的精密度(RSD)为3.27%,回收率为100.7%。研究首次获得HPLC法与Jensen分光光度法的关系式y=8.496x-12.237(r=0.9991)。研究开展了雨生红球藻BBM培养...Astaxanthin is the main carotenoid pigment in aquatic animals and is widespread in nature world. The fresh-water green unicellular alga Haematococcus pluvialis (Chlorophyceae) has become a focus due to its ability to accumulate the high-value pigment astaxanthin. It is generally accepted that Haematococcus pluvialis can accumulate the highest levels of astaxanthin (1-3% by dry weight) among the na...学位:理学硕士院系专业:海洋与环境学院海洋学系_海洋化学学号:20032701

    Optimization for astaxanthin extraction from Haematococcus pluvialis with ethanol using response surface methodology

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    采用安全无毒的乙醇为溶剂,研究了超声时间、料液比、搅拌速度等因素对虾青素提取效率的影响。在此基础上,采用响应面设计对以上3个因素的交互关系进行探究。结果表明,3个因素对虾青素提取率的影响顺序为:超声时间〉料液比〉搅拌速度;最佳提取条件为:超声时间20min、料液比1:150、搅拌速度1000 r/min,在此条件下虾青素提取率可达48.42%。Ethanol, an environmentally friendly solvent, was used to extract astaxanthin from Haematococcus pluvialis. The effect of several conditions, including ultrasound time, the ratio of solid to liquid (S/L) and stirring rate, on astaxanthin extraction rate was investigated. Response surface methodology was applied to study the interaction in these factors. The effects of three factors on the extraction of astaxanthin were in order of ultrasound time, S/L, and stirring rate. The optimal astaxanthin extraction rate was 48.42% with 20 min of ultrasound time, 1:150 of S/L, and 1000 r/min of stirring rate.国家自然科学基金项目(41606177); 福建省高校产学合作项目(2015N5006); 福建省教育厅中青年教师教育科研项目(JA15406); 泉州市科技局校地协同创新项目(2016N056

    Exploration for effects of acoustic stimulus on movement behavior and HSP70 expression of two kinds of marine crustaceans

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    水下噪声会潜在的引起海洋生物紧张,分散和掩蔽这些海洋生物重要的声信号,进而对海洋生物的行为和生存造成影响。近几十年来,随着人类社会的不断发展,人们将发展目光投向了占有全球71%面积的海洋。伴随海洋事业的快速发展,海洋工程噪声对海洋生物的影响引起了人们的极大关注。虽然目前已有不少研究成果评估了水下工程噪声对海洋生物的影响,这些噪声包括轮船、打桩、地震勘探以及风电场等水下噪声,表明海洋工程等人为噪声对鱼类以及海洋哺乳动物都将造成了不同程度的影响和伤害。但是,有关评估声刺激对海洋食物链中不可或缺的海洋甲壳动物影响的研究还少有报道。 海洋甲壳动物种类繁多,且多是重要的食用种,具有极大的经济价值。其中...Underwater noise has the potential to bring stress to marine animals, distract and mask important sounds, and thus affect marine animal behavior and physiological parameters. Along with the development of human society, the human has given a new focus on exploring ocean resources. In recent decades, with the rapid development of the marine industry, the effect of man-made noises on marine life has...学位:理学硕士院系专业:海洋与地球学院_海洋物理学号:2232013115144

    Medium Optimization of Astaxanthin by Oceanic Red Yeast

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    为了提高海洋红酵母发酵虾青素的产量水平,对海洋红酵母的培养基成分进行了初步研究。试验结果表明,海洋红酵母能利用葡萄糖、淀粉水解糖、糖蜜等多种碳源,用淀粉水解糖为碳源培养海洋红酵母所获得的虾青素体积产率最大;用牛肉膏为氮源有利于提高海洋红酵母的生物量,以(NH4)2SO4、NH4Cl和蛋白胨为氮源有利于提高海洋红酵母的虾青素体积产率,用KNO3、草酸铵、蛋白胨、尿素有利于提高海洋红酵母的虾青素细胞产率;在海洋红酵母的培养基中添加Mn2+、Cd2+、Zn2+、Fe2+能增加生物量,添加Zn2+、Fe3+、Mn2+能增加海洋红酵母的虾青素体积产率,添加Fe3+能提高海洋红酵母的虾青素细胞产率。In order to improve the production level of astaxanthin by oceanic red yeast(ORY),the ingredients of medium for it were studied.The results of experiment showed that ORY could utilize many kinds of carbon sources such as glucose,starch hydrolyzed sugar(SHS),and molasses etc.However,volume productivity of astaxanthin was the biggest among these sugars when using SHS.Using beef extract as nitrogen source is helpful to enhance biomass of ORY, while(NH4)2SO4,NH4Cl and peptone were conducive to improve the ORY volume productivity of astaxanthin.In addition,KNO3,ammonium oxalate,peptone,and urea were conducive to improve the cellular productivity of astaxanthin.Adding Mn2+,Cd2+,Zn2+,and Fe2+ to the ORY medium,could increase the biomass.Adding Zn2+,Fe2+,and Mn2+ could increase the ORY volume productivity of astaxanthin.And adding Fe2+ could increase the ORY cellular productivity of the astaxanthin.福建省重点科技项目(2006H0033);; 厦门市科技项目(B04066

    Photoprotection Mechanisms and Crosstalk between the Biosynthesis of Fatty Acids and Astaxanthin in Haematococcus Pluvialis

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    虾青素是一种重要的类胡萝卜素,具有良好的着色功能、超强的抗氧化功能及抗肿瘤作用,在水产养殖、食品添加剂、医药保健品等方面有着广泛的应用前景。单细胞雨生红球藻(Haematococcuspluvialis)在胁迫条件下能够积累大量的虾青素(约为细胞干重的4%)和中性脂肪酸(约为细胞干重的25%)。因此,雨生红球藻被认为是天然虾青素最好的来源。 雨生红球藻除了是虾青素等次级类胡萝卜素的最好天然来源外还是一种很有前景的生物柴油来源。生长迅速的雨生红球藻运动细胞常被用作在各种胁迫条件下生产虾青素和TAG的工作细胞。然而,由于运动细胞对胁迫环境比较敏感,诱导初期细胞死亡率高,使细胞生物量和目标产物产量...Astaxanthin, an important carotenoid, has been increasingly used in aquaculture industry, food additives, medicine and health products because of its color feature, excellent antioxidant activity, anti-tumor function. The green microalga Haematococcus pluvialiscan synthesize significant amounts of astaxanthin (~ 4% of dry cell weight, DCW) and storage neutral lipids (~25% of DCW) under adverse env...学位:工学博士院系专业:化学化工学院_工业催化学号:2062010015362
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