1,815 research outputs found

    Bio-imaging with a group of acid-responsive fluorescence-on probes

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    水作为所有细胞和组织的重要组成部分,其pH和诸多生理活动密切相关.荧光分析具有可视化、高分辨率等特点,在生命体系的pH分析中得到广泛应用.近年来; , pH触发荧光成像技术在生物医学领域逐渐得到应用.本文简要介绍了一类酸度触发荧光、含分子内螺环的罗丹明衍生物的设计与功能化,及其生物成像应用.pH is critical for diverse physiological activities. Disruption of pH; homeostasis is pertinent to myriad pathological events. Particularly,; the organelle-specific pH within mammalian cells is elaborately; controlled and yet succumbs to pathological stress. Lysosomes are the; major intracellular acidic compartments, and the lysosomal acidity is; critical for diverse biological events. Aberrant lysosomal pH is; manifested a broad spectrum of diseases ranging from inflammation to; cancer metastasis, and to lysosomal storage diseases. As such, defining; pH changes in specific pathological settings would be of use for; biomedical investigations. Rhodamine-lactams, a group of nonfluorescent; rhodamine derivatives featuring intramolecular spirolactam, are poised; to proton triggered opening of the lactams to give fluorescent; rhodamines, and has been widely used for lysosomal imaging in cell; models. Herein we review the use of structurally modified; rhodamine-lactams as acidity-reporting entities for bioimaging of; distinct biological events, including host-pathogen interaction, cell; death, autophagy, and mitochondrial depolarization in cells, and; inflammation and tumors in mice models.国家自然科学基金; 福建省自然科学基金; 中央高校基本科研业务费专项资

    The molecular probes based on chemodosimeter for DCP,Cu+ and HClO

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    荧光分子探针即是可以将物质的化学信息转换为可测量的荧光信号的一种光学传感器,它具有选择性好、灵敏度高、操作简便、实时快速检测等优点。随着相关技术的成熟,将荧光分子探针与细胞显微成像结合的技术已经成为了人类探测微观生命系统的一种重要手段。本文在前人研究的基础上,成功设计合成了三种荧光分子探针,体现了不同的检测性能。本论文共分四章,分别包括以下内容: 第一章,绪论。首先介绍了光学分子传感器的基本概念、研究现状和发展趋势,着重介绍了荧光分子探针的设计机理;其次重点介绍了神经毒剂类似物二乙基磷酰氯、亚铜离子和次氯酸荧光分子探针的研究现状和发展趋势;最后,通过结合相关文献的研究内容及本课题组工作成...Fluorescence molecular probe is a kind of optical sensor which can transform the chemical information of substance into measurable fluorescent signal. It has the advantages of good selectivity, high sensitivity, simple operation and rapid detection in real time. And the technology of combining fluorescence molecular probe with the cell microscopic imaging has become an important means of studying ...学位:理学硕士院系专业:化学化工学院_分析化学学号:2052012115146

    Application of rhodamin-based acid sensitive fluorescent probe in cell biology and tumor diagnosis

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    荧光分子探针具有特异性、专一性、灵敏性、快速便捷以及可视化等优点,近年来逐渐在环境监测、生命科学研究、医学诊断等领域得到重视。尤其是在生物医学领域,人们对于应用荧光探针来检测细胞内环境的变化以及实现肿瘤的可视化诊断越来越有兴趣。细胞内或肿瘤内环境具有特殊的性质,如溶酶体的pH要比其他细胞内囊泡的pH更低,肿瘤的pH比正常组织更低。利用这一性质可以为肿瘤的早期诊断提供思路。本文总结了现有的研究成果,设计了新的酸敏感的荧光分子探针R6G-EDA,基于该探针设计了一系列测定胞内酸度的纳米颗粒,并研究了酸敏感纳米颗粒在肿瘤诊断中的应用。 一、基于罗丹明衍生物“开关环”机理的酸敏感探针R6G-EDA ...Fluorescence molecular probe has advantages such as specificity, specificity, sensitivity, fast, convenient and visualization. In recent years, people pay great attention to its application in environmental monitoring, medical diagnostics, and other fields. Especially in the biomedical field, people have great interests for the application of fluorescent probe to detect cellular changes in the env...学位:理学博士院系专业:化学化工学院_生物化工学号:2052011015370

    Nano Sensors for Oxygen Based on Ratiometric Fluorescence

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    比率式荧光传感通过在同一传感基质中掺杂两种或两种以上对分析物具有不同响应的发光体,根据不同的光发射信号比值构建起来的光化学传感体系,能更好满足实际检测的需要。近年来,比率式荧光纳米传感器的构建已引起了人们的广泛关注。该文介绍了比率式荧光纳米氧传感器的制备方法、传感特性和应用情况,并对其传感检测的发展和应用前景进行了分析和展望。Ratiometric sensing nanoprobes derivatized from luminescent dyes show great application in a nano or micro system as well as in a sensing material. Nowadays,a ratiometric nano sensing system has attracted a wide variety of attentions. In this review,the meaning of measuring oxygen concentration was introduced and the determination approaches for O_2 was discussed. The research progress of nanosensors for O_2 based on ratiometric fluorescence including their preparation,characteristics and applications,was systematically reviewed.国家自然科学基金资助项目(21375112

    Activatable Ferritin Nanocomplex for Real-Time Monitoring of Caspase-3 Activation during Photodynamic Therapy(PDT)

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    随着分子影像学的发展,治疗诊断学已成为现在医学研究的热点。目前各种 先进的医学成像技术已被用在医学成像,例如光学成像,磁共振成像(MRI),单 光子发射计算机断层扫描(SPECT),正电子发射断层扫描(PET)和光声成像, 从而促进了分子成像探针和医学成像的发展。分子成像探针结构复杂,能够结合 特定生物标志物并产生成像信号用于疾病的诊断。迄今为止,一些成像探针已应 用到临床,例如18F-FDG,但是探针的低靶向性特点限制了成像探针的应用。因 此,开发由特定的靶向基团和成像配基组成的高度敏感的可活化探针是迫切需要 的,并引起了科学家的广泛关注。最有潜力的可活化探针结构包括酶的底物,...Theranostics has become a hot topic in current medical research with the rapid develepment of medical imaging. Nowadays variety of advanced medical imaging technologies have been utilized in medical imaging, for instance optical imaging, magnetic resonance imaging (MRI), emission tomography (PET), single-photon emission computed tomography (SPECT) and photoacousti...学位:医学硕士院系专业:公共卫生学院_流行病与卫生统计学学号:3272013115055

    Several Original Applications of Water-soluble Phthalocyanines as Optical Probes in Analytical Sciences

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    酞菁化合物大多性质稳定、易于合成、结构可修饰性强,可引入各类活性基团从而获得具有各种功能的衍生物。尤为难得的是,中心配位原子为反磁性的金属酞菁化合物大多具有荧光量子产率高、荧光发射在红色区域且可进行长波激发(因而可有效避开背景荧光和散射光的干扰)等优良荧光特性。本学位论文围绕金属酞菁作为光学分子探针在分析科学的应用而展开,共六章。 第一章首先对酞菁的结构、基本性质与应用作了简要介绍。重点阐述酞菁在分析科学领域的应用,包括酞菁在催化动力学分析中的应用、酞菁作为光学探针的应用以及酞菁作为光学成像探针的应用等。 第二章建立了铋离子的高特异性、高灵敏的定量分析新方法,并对反应机理进行了探讨。在酸性...Phthalocyanine compounds are stable compounds with facile way of synthesis. The parent structure of phthalocyanines can be modified by introducing active groups to obtain a variety of functional derivatives. Intrestingly, when antimagnetic metal ions coordinated with phthalocyanien, the resulting metal phthalocyanines compounds turn to be fluorescent with high quantum yield. Fluorescent phthaloc...学位:医学硕士院系专业:医学院_药理学学号:2452013115355

    量子点荧光探针在生物医学研究中的应用进展

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    20世纪末,随着纳米科技的进步,诞生了一类新型荧光探针—量子点.与传统有机荧光探针相比表现出独特而优良的发光特性,在不到10a的时间里,这类探针在科学研究中得到了迅速的应用.本文对量子点荧光探针在生物医学中的应用及进展进行简要的综述

    Study on the Water-soluble Porphyrin and Phthalocyanine Optical Probes for the Detection of pH and the Quantitative Analysis of Hg(II)

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    卟啉是一类由四个吡咯亚基的α-碳原子通过次甲基桥(=CH-)互联而形成的杂环化合物,由于其侧链的差异而形成大量衍生物,且分布广泛。卟啉分为两大类:脂溶性和水溶性卟啉化合物。后者因在水介质中有较好的溶解度长期以来在分子识别、分析化学、电化学及光化学反应等化学领域获得了广泛应用,在生物医学领域的应用也越来越令人瞩目。 血卟啉及其衍生物具有优良的光化学、光物理性质,自上世纪70年代起即作为新型光化学诊治剂得到广泛的研究与应用;其在生物医学、催化、仿生学、分析化学、环保等领域中的应用也得到逐步开拓。本论文主要研究其作为pH探针在酸度测定中的应用。 酞菁是母体结构与卟啉类似的大环类化合物,性质稳定、...Porphyrins are macro-heterocyclic compounds formed by interconnection of α-carbon atoms of four pyrrole subunits through a methine bridge (=CH-). There are a variety of porphyrin compounds, due to the differences in the side chains. Commonly, porphyrins are divided into two classes by solubility, i.e., lipophilic porphyrins and water-soluble porphyrins. Water-soluble porphyrin has been widely u...学位:医学硕士院系专业:医学院_药理学学号:2452014115358

    Development in detection of enterohemorrhagic Escherichia coli O 157 and H 5 subtype avian influenza virus using real-time fluorescence PCR

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    实时荧光PCR技术具有高灵敏度、高特异性、无需PCR反应后处理步骤等优点,近年来被广泛应用于分子生物学研究领域,尤其在临床检验方面,该技术提供了有力的支持。针对日益完善的实时荧光PCR技术和种类越来越多的荧光探针技术,本研究设计了一种新型的荧光探针——等长双链荧光探针,又称Y型荧光探针,并围绕该关键技术展开了研究。研究工作主要包括Y型荧光探针的设计组合,应用Y型荧光探针对常见的病原微生物进行实时定量的检测。文章分为两部分。在第一部分,首先,我们对不同组合的Y型荧光探针进行了筛选、优化,找到最佳的检测探针组成。然后,我们以肠出血性大肠杆菌O157为检测目标,以该细菌的rbfE基因为检测靶序列,从...Recently, real-time polymerase chain reaction (RT-PCR) has been widely used in the research of molecular biology, especially in clinic diagnosis, because of its high specificity, sensitivity and without any further processing of the samples and opening the test tubes. This dissertation consists of two parts. The first part is the development of a new fluorescence probe-equal-length double-stran...学位:理学硕士院系专业:生命科学学院生物学系_细胞生物学学号:20022606
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