室内植物表型平台及性状鉴定研究进展和展望

Plant phenomics is under rapid development in recent years, a research field that is progressing towards integration, scalability, multi-perceptivity and high-throughput analysis. Through combining remote sensing, Internet of Things (IoT), robotics, computer vision, and artificial intelligence techniques such as machine learning and deep learning, relevant research methodologies, biological applications and theoretical foundation of this research domain have been advancing speedily in recent years. This article first introduces the current trends of plant phenomics and its related progress in China and worldwide. Then, it focuses on discussing the characteristics of indoor phenotyping and phenotypic traits that are suitable for indoor experiments, including yield, quality, and stress related traits such as drought, cold and heat resistance, salt stress, heavy metals, and pests. By connecting key phenotypic traits with important biological questions in yield production, crop quality and Stress-related tolerance, we associated indoor phenotyping hardware with relevant biological applications and their plant model systems, for which a range of indoor phenotyping devices and platforms are listed and categorised according to their throughput, sensor integration, platform size, and applications. Additionally, this article introduces existing data management solutions and analysis software packages that are representative for phenotypic analysis. For example, ISA-Tab and MIAPPE ontology standards for capturing metadata in plant phenotyping experiments, PHIS and CropSight for managing complicated datasets, and Python or MATLAB programming languages for automated image analysis based on libraries such as OpenCV, Scikit-Image, MATLAB Image Processing Toolbox. Finally, due to the importance of extracting meaningful information from big phenotyping datasets, this article pays extra attention to the future development of plant phenomics in China, with suggestions and recommendations for the integration of multi-scale phenotyping data to increase confidence in research outcomes, the cultivation of cross-disciplinary researchers to lead the next-generation plant research, as well as the collaboration between academia and industry to enable world-leading research activities in the near future

Metallic Nanofilm Mediated Fluorescence Techniques for Cell Research

金属纳米薄膜具有调控荧光团发光性质的特点，吸引了越来越多研究者的关注。它主要通过两种方式对荧光团的发光性质进行调控。1）金属纳米薄膜表面的等离子体与荧光团发生耦合，使之发射高度定向的、偏振的荧光信号，产生表面等离子体耦合发射荧光（SurfacePlasmonCoupledEmission，SPCE）。由于表面等离子体共振（SPR）模式和等离子体波导共振（PWR）模式的存在，SPCE技术的耦合距离可从纳米级拓展到微米量级，在微米级尺度样品的研究中展现出应用潜力。2）金属纳米薄膜反射激发光和发射光，反射光束与原光束发生干涉，产生镜面效应，从而改变荧光团的发光强度、荧光寿命和发射空间分布。镜面效应具...Metallic nanofilms have attracted more and more attention owing to their characteristics in engineering the emission properties of fluorophores. Generally, there are two manners of engineering: the first is surface plasmon coupled emission (SPCE). The excited fluorophores couple with surface plasmons on metal surface and result in highly directional and polarized emission. The coupling distance of...学位：理学博士院系专业：化学化工学院_分析化学学号：2052008015016

荧光偏振发射平台法及其应用

本文从原理上叙述荧光偏振发射平台法,并用荧光单体-单体混合物及单体-聚合物体系进行验证试验。前者如荧光黄-罗丹明S(或四碘荧光素、或罗丹明B),后者如罗丹明6G-罗丹明6G聚合物体系,结果满意

UVA Assisted 4-Thiothymidine for Cancer Treatment

This article reviews the developments of 4-thiothymidine analogues, assisted with UVA light, as a novel cancer therapy. First, the key points on synthetic chemistry, photochemistry and cellular toxicity of 4-thiothymidine are summarized. As the chemical structure of 4-thiothymidine is very similar to that of its parent thymidine, thus 4-thiothymidine can be readily incorporated into cellular DNA, and with the help of thymidine kinase, much more preferably into cancerous DNA. Unlike thymidine, 4-thiothymdine can absorb strongly in UVA (longer wavelengths of UV) light. Thus UVA-assisted 4-thiothymidine offers an effective cancer treatment. Some underlying mechanisms of action by 4-thiothymidine/UVA and compares this cancer approach with the commonly used photodynamic therapy are discussed. The various interactions between 4-thiothymidine with human serum albumin are introduced. Finally, a short conclusion on the past efforts and a brief prospect for future work in this exciting research field are given

三维荧光光谱总体积积分法

三维荧光光谱法是近20年来发展起来的荧光分析新技术.我们利用其丰富的信息量,提出的三维荧光光谱总体积积分法不仅较好地体现了三维荧光光谱选择性高的特点,而且灵敏度较常规法有较大的提高,从而进一步体现了三维荧光光谱的优越性.本文拟通过一系列荧光物质的检测,进一步探讨该方法的特点

胶束增敏酶联免疫吸附法荧光测定人IgG及与均相法测定比较

在荧光免疫分析中,胶束的加入常引起荧光强度增大、光谱位移、荧光寿命和荧光偏振的变化,利用这些变化可以改进自由抗原(Ag)和抗体(Ab)结合抗原的识别,提高分析灵敏度,扩大荧光免疫分析的范围。我们曾详细研究了环境介质对酶联免疫吸附测定(ELISA)荧光指示反应体系的影响,观察到长链烷烃季铵盐胶束对反应兼有催化和增敏作用。本文报导了将胶束增敏作用引入ELISA荧光测定体系,探求解决该体系酶促反应与荧光检测独立进行问题及提高灵敏度的途径,并与作者新近报导的一种均相荧光酶免疫分析法进行了比较

Preparation of Europium-Containing Silicon Oxynitride Phosphor doped by Magnesium with High Quantum Efficiency

利用聚合物先驱体转化法可以成功制备物化性能稳定、相结构简单的含铕硅基氮氧化物荧光粉，但其量子效率不佳，导致发光强度不够高。适量镁掺杂可以显著提高稀土掺杂碱土金属硅基氮氧化物以及铈掺杂硅基氮氧化物荧光粉的发光性能，但在聚合物先驱体转化法中掺镁对含铕硅基氮氧化物荧光粉的影响尚不清楚，因此有必要探索通过掺镁制备结构简单且具有高量子效率的含铕纯硅基氮氧化物荧光粉。本论文探讨采用聚合物先驱体转化法，以聚碳硅烷、乙酰丙酮铕和乙酰丙酮镁为原料来制备高量子效率的掺镁含铕硅基氮氧化物荧光粉。结合元素分析、XRD、SEM及荧光光谱，系统地探究了烧结方法、终烧温度和投料比对荧光粉的组成、相结构、形貌及荧光性能的影响...The europium (Eu)-doped silicon oxynitride phosphor with stable physicochemical properties and simple phase structure has been successfully synthesized via a novel polymer-derived method. However, the photoluminescence properties (quantum efficiency) of those phosphors need to be further improved. It has been found that doping with appropriate amount of magnesium (Mg) ion could highly enhance the ...学位：工程硕士院系专业：材料学院_工程硕士(材料工程)学号：2072014115011

Preparation andProperties of Fluorescent Gold Nanoclusters Functionalized Silk Biomaterials

家蚕蚕丝作为天然的蛋白质纤维，具有独特的力学性能、良好的生物相容性和可控的生物降解性，已被作为生物医学理想材料。然而，蚕丝材料作为生物医学材料植入体内后，进行体内的检测和追踪仍是目前一大难点。本研究通过共混荧光金纳米簇和丝素蛋白，制备可用于体内荧光成像的蚕丝材料（3维支架、薄膜、凝胶），为生物材料的体内成像提供了思路。本研究主要内容如下： 冷冻干燥法制备三维多孔荧光丝素蛋白/金簇复合支架。复合支架具有红色荧光，研究发现丝素蛋白/金簇混合溶液的pH会影响金簇的荧光强度，发射峰位和荧光效率，支架荧光量子产率可达18%。金簇的加入增加丝素蛋白β-折叠和结晶度。丝素蛋白/金簇复合支架的孔洞之间相互贯...Bombyx mori silk fibers, as a natural protein, have been adsorbing great interests in the fields of biomedicine, due to robust mechanical properties, excellent biocompatibility and controllable biodegradation. However, the monitoring of the evolution of the overall structure and organization of the materials in vivo or in vitro remains as a neck bottle for the further refinement of silk bio-materi...学位：理学硕士院系专业：材料学院_生物医学工程学号：3142013115012

Preparation and Photoluminescence of Praseodymium-Doped Silicon Aluminum Oxynitride Phosphors by Polymer-Derived Method

应用先驱体转化法制备稀土掺杂硅基氮氧化物荧光粉，可以改善传统的固相反应法所需要的高温高压等苛刻条件。掺镨含铝硅基氮氧化物红色荧光粉具有较好的发光稳定性及化学稳定性，在显示照明领域具有潜在的应用价值。目前，尚没有先驱体转化法制备掺镨含铝硅基氮氧化物红色荧光粉的报道，因此，有必要探讨先驱体转化法制备该荧光粉的合成工艺及参数。本论文采用聚碳硅烷、乙酰丙酮镨和乙酰丙酮铝为原料，通过聚合反应得到先驱体，在氨气中经氮化实现有机-无机转化，再经结晶制备掺镨含铝硅基氮氧化物荧光粉。系统研究了烧结温度、投料原子比、保温时间和分步研磨处理对掺镨含铝硅基氮氧化物荧光粉相结构、化学组成和发光性能的影响。 结果发现，...Such harsh conditions as high temperature and high pressure required to prepare rare-earth-doped silicon based oxynitride phosphors through conventional solid-state reaction method can be much improved by polymer-derived method. The praseodymium (Pr)-doped silicon aluminum oxynitride (SiAlON) red phosphors possess excellent luminescence properties and chemical stability. They have potential applic...学位：工程硕士院系专业：材料学院_工程硕士(材料工程)学号：2072013115011