The Research on the Interaction Domains among Three Major envelope Proteins and their Interaction with Chitin

对虾白斑综合症病毒（WSSV）是严重阻碍对虾养殖业健康发展的主要病害之一。WSSV是一种无包涵体、具双层囊膜、杆状型的双链环状大型DNA病毒，其基因组大约为300kbp，隶属于线形病毒科(Nimaviridae)白斑病毒属（Whispovirus）。WSSV宿主范围广，能对对虾和多种甲壳动物造成高致病性、高死亡率的感染，然而病毒侵染宿主的机制至今仍不清楚。在本研究中，我们鉴定了病毒3种主要囊膜蛋白VP24、VP26和VP28之间的相互作用位点。此外，我们也首次发现WSSV可以与几丁质结合，且VP24在结合过程中起关键作用。本研究工作的内容主要包括以下几部分： （1）通过构建VP24和VP28...White spot syndrome virus （WSSV）is one of the major pathogens that seriously obstructed the development of the shrimp acquclture. It has caused enormous economic losses to shrimp farming industry since 1993. The WSSV is a large, nonoccluded, enveloped, rod- or elliptical-shaped, doubled-stranded DNA virus of approximately 300 kbp and it belongs to the Nimaviridae family. As a major pathogen in the...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162012115232

Screening of signal transducting molecules involved in the infection of White spot syndrome virus

对虾为水产养殖产业中的重要经济品种，每年为人们带来大量的海鲜食材并创造了巨大的经济效益。但近年来不断的病害尤其是对虾白斑综合症病毒（White spotsyndromevirus，WSSV）导致对虾大量减产，造成的直接或间接的经济损失十分巨大。研究对虾先天性免疫机制对于对虾抗病害十分重要。 本实验室收集了365种对细胞信号转导分子具有调节功能的化合物，在高等哺乳类如人和小鼠细胞中已经通过使用这些化合物对其靶基因激活/抑制即控制信号通路的开关来研究信号通路的功能。本实验室在此方法基础上，分离螯虾造血组织并使用胶原酶制备单细胞悬液，将这些化合物处理细胞并人工感染WSSV病毒粒子，提取螯虾造血干...Shrimp is one of the important species of the aquaculture industry. But, many virulent pathogens especially the White spot syndrome viru (WSSV) has caused a high shrimp mortality and great economic losses. So, it is crucial to study the shrimp immune system and breed the virus-resistant shrimp. We collected 365 molecules which could modulate the signal pathway transduction. These molecules h...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162013115258

Research on the interaction between WSSV immediate-early protein WSV056 or IE1 and retinoblastoma family protein

对虾白斑综合症病毒（whitespotsyndromevirus，WSSV）是一种具有囊膜的、无包涵体的、类杆状双链环状DNA病毒，是危害对虾养殖的主要病原之一，对世界对虾养殖业造成了严重的损失。WSV056和IE1是对虾白斑综合症病毒的极早期蛋白，它们在病毒进入宿主细胞的4小时内即可开始表达，并且与宿主细胞的某些调控蛋白相互作用，为病毒在宿主细胞内的增殖做准备。WSV056和IE1在基因序列和蛋白结构上有着很高的相似性。本论文围绕WSV056和IE1开展研究，具体包括以下几个方面：1.通过酵母双杂交技术，在酵母AH109细胞内验证了IE1与RB的结合，同时在昆虫细胞highfive细胞内验证...White spot syndrome virus （WSSV）, an enveloped, non-occluded, bacilliform and circular dsDNA virus, is one of the major pathogens of shrimp diseases, causing considerable mortality in penaeid shrimp. Both WSV056 and IE1 are viral immediate-early (IE) proteins, which begin producing in only 4 hours after the virus enter the host cell. What’s more, they can interact with some proteins in the cell,wh...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2172009115208

Functional Analysis of Envelope Protein VP33 and Identification of non-structure protein wsv406 of White Spot Syndrome Virus

对虾白斑综合症病毒(whitespotsyndromevirus，WSSV)是危害全球对虾养殖业的主要病毒病原之一。它是一种具有囊膜的、无包涵体的、类杆状双链环状DNA病毒，具有传染力强，致死率高，难防治的特点。由于其宿主范围广泛，能够侵染多种甲壳纲动物，对养殖业和海洋环境都构成了严重威胁。本论文主要针对病毒结构蛋白开展研究。这有利于揭示病毒复制过程中的组装机制，从而为最终建立有效的防治方法提供有力的科学依据。研究工作主要分为两部分：（1）膜蛋白VP33的鉴定。之前的研究通过对完整病毒的结构蛋白进行SDS-PAGE电泳和质谱鉴定后，结合氨基酸序列比对分析，已确定VP33为WSSV基因组中ORF...Shrimp white spot syndrome virus (WSSV) is a pathogen causing heavy mortality in shrimp farms throughout the world, which is known to be the most critical shrimp disease known until now. Virion is a rod-shaped, tightly covered with three-layered envelope and has a tail-like appendage at its end. The WSSV genome consists of a double-stranded DNA estimated to be well over 200 kb in size. This virus ...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2012005130213

Preliminary identification of Envelope Protein VP32 and Collagen-like protein CLP of White Spot Syndrome Virus

对虾白斑综合症病毒(whitespotsyndromevirus，WSSV)是一种具有囊膜、无包涵体的杆状型双链环状DNA病毒，Nimaviridae属Whispovirus种中唯一的物种，也是严重危害对虾养殖业的最主要病原。 目前我们的研究主要围绕WSSV的蛋白功能开展研究。这是因为病毒蛋白参与病毒的形态构成、对宿主细胞的入侵、增殖等一系列活动，并在其中扮演了重要角色。 本论文研究分为以下两个部分： （1）质谱发现病毒膜蛋白VP32是ORFwsv198的编码产物。我们构建了融合表达载体pGEX-2Th-vp32和pMAL-vp32并分别在E.coliBL-21菌株中表达纯化了GST-V...White Spot Syndrome Virus (WSSV) is a large, rod-shaped, enveloped double-stranded DNA virus. The WSSV has been classified to be the sole species of Whispovirus in genus of Nimaviridae, and the main pathogen harming shrimp industry. At present, our research mainly focuses on the main proteins of WSSV. These proteins play important roles in the formation construction, invasion, proliferation, etc...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：2012005130206

Identification and Characterization of White Spot Syndrome viral immediate-early genes

对虾白斑综合症病毒（WhiteSpotSyndromeVirus，WSSV）是危害对虾养殖业的最主要病原，它是一种具有囊膜、无包涵体的杆状型双链环状DNA病毒。病毒极早期基因编码的调控蛋白在病毒的感染过程中起着至关重要的作用。本文阐述了在体外原代培养的螯虾血淋巴细胞中WSSV基因转录和基因组复制的情况。前期的研究工作成功的构建了基于原代螯虾血淋巴细胞体外培养的WSSV感染模型；利用WSSV全基因组芯片杂交技术对用蛋白抑制剂-放线菌酮（CHX）处理的原代培养的螯虾血淋巴细胞的样品进行WSSV极早期基因的筛选。本文对芯片筛选得到的候选基因进行RT-PCR鉴定和转录时相分析，最终确定了16个WSSV...White Spot syndrome virus (WSSV) is the major viral pathogen of shrimp industry, which is kind of enveloped, no inclusion stage, rod-shaped, double-strand DNA virus. During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. This thesis demonstrated WSSV genes’ expression and genome replication in primary cultured crayfish hemocyte in vit...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2162007115192