3,737 research outputs found

    Catalogue of the Hantken collection: carbonate microfacies photographs from 1872-82

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    Maximilian Hantken (1821-1893), founding professor of the Department of Palaeontology at Budapest University, was a pioneer in stratigraphic micropalaeontological studies. He assembled a collection of microphotographs of Mesozoic and Cenozoic carbonate rocks from Hungary and Italy for educational purposes. A catalogue of the 232 photos, mounted on wooden boards or cardboard is given here. The photos date back to the decade between 1872 and 1882, as shown by newspaper cuts pasted on the backsides

    Draft Genome Sequence of an Escherichia coli O157:H43 Strain Isolated from Cattle

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    Here we report the draft genome sequence of an Escherichia coli O157:H43 strain, designated T22, with an atypical virulence gene profile and isolated from healthy cattle. T22 produces cytolethal distending toxin V (CDT-V) and belongs to phylogenetic group B1 and sequence type 155 (ST155)

    Sequence Variability of P2-Like Prophage Genomes Carrying the Cytolethal Distending Toxin V Operon in Escherichia coli O157

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    Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and strains not fitting any established pathotypes. In this study, we were the first to sequence and annotate a 31.2-kb-long, noninducible P2-like prophage carrying the cdt-V operon from an stx- and eae-negative E. coli O157:H43 strain of bovine origin. The cdt-V operon is integrated in the place of the tin and old phage immunity genes (termed the TO region) of the prophage, and the prophage itself is integrated into the bacterial chromosome between the housekeeping genes cpxP and fieF. The presence of P2-like genes (n = 20) was investigated in a further five CDT-V-positive bovine E. coli O157 strains of various serotypes, three EHEC O157:NM strains, four strains expressing other variants of CDT, and eight CDT-negative strains. All but one CDT-V-positive atypical O157 strain uniformly carried all the investigated genomic regions of P2-like phages, while the EHEC O157 strains missed three regions and the CDT-V-negative strains carried only a few P2-like sequences. Our results suggest that P2-like phages play a role in the dissemination of cdt-V between E. coli O157 strains and that after integration into the bacterial chromosome, they adapted to the respective hosts and became temperate

    Einführung. Die heutige Konnotation des schwäbischen Phänomens.

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    A bevezető az MTA-DE Néprajzi Kutatócsoport és a DE Néprajzi Tanszék "Die heutige Konnotation des schwäbischen Phänomens" című konferenciájának tematikáját mutatja be, különös hangsúlyt fektetve a sváb fogalom jelentéstartalmaira, valamint kitér a fent nevezett intézmények ide vonatkozó sváb kutatásaira is

    The long polar fimbriae (lpf) operon and its flanking regions in bovine Escherichia coli O157:H43 and STEC O136:H12 strains

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    Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain from bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contain only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, but its role in adherence might be masked by other uncharacterized adhesins

    PROBLEME DER ZEITGEMÄSSEN BEMESSUNG VON FÖRDERTECHNISCHEN MASCHINEN

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