129,635 research outputs found

    Bio-inspired pulmonary surfactant-modified nanogels : a promising siRNA delivery system

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    Inhalation therapy with small interfering RNA (siRNA) is a promising approach in the treatment of pulmonary disorders. However, clinical translation is severely limited by the lack of suitable delivery platforms. In this study, we aim to address this limitation by designing a novel bioinspired hybrid nanoparticle with a core-shell nanoarchitecture, consisting of a siRNA-loaded dextran nanogel (siNG) core and a pulmonary surfactant (Curosurf (R)) outer shell. The decoration of siNGs with a surfactant shell enhances the colloidal stability and prevents siRNA release in the presence of competing polyanions, which are abundantly present in biofluids. Additionally, the impact of the surfactant shell on the biological efficacy of the siNGs is determined in lung cancer cells. The presence of the surfactants substantially reduces the cellular uptake of siNGs. Remarkably, the lowered intracellular dose does not impede the gene silencing effect, suggesting a crucial role of the pulmonary surfactant in the intracellular processing of the nanoparticles. In order to surmount the observed reduction in cellular dose, folate is incorporated as a targeting ligand in the pulmonary surfactant shell to incite receptor-mediated endocytosis. The latter substantially enhances both cellular uptake and gene silencing potential, achieving efficient knockdown at siRNA concentrations in the low nanomolar range. (C) 2015 Elsevier B.V. All rights reserved

    Optical Spectroscopy and Nebular Oxygen Abundances of the Spitzer/SINGS Galaxies

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    We present intermediate-resolution optical spectrophotometry of 65 galaxies obtained in support of the Spitzer Infrared Nearby Galaxies Survey (SINGS). For each galaxy we obtain a nuclear, circumnuclear, and semi-integrated optical spectrum designed to coincide spatially with mid- and far-infrared spectroscopy from the Spitzer Space Telescope. We make the reduced, spectrophotometrically calibrated one-dimensional spectra, as well as measurements of the fluxes and equivalent widths of the strong nebular emission lines, publically available. We use optical emission-line ratios measured on all three spatial scales to classify the sample into star-forming, active galactic nuclei (AGN), and galaxies with a mixture of star formation and nuclear activity. We find that the relative fraction of the sample classified as star-forming versus AGN is a strong function of the integrated light enclosed by the spectroscopic aperture. We supplement our observations with a large database of nebular emission-line measurements of individual HII regions in the SINGS galaxies culled from the literature. We use these ancillary data to conduct a detailed analysis of the radial abundance gradients and average HII-region abundances of a large fraction of the sample. We combine these results with our new integrated spectra to estimate the central and characteristic (globally-averaged) gas-phase oxygen abundances of all 75 SINGS galaxies. We conclude with an in-depth discussion of the absolute uncertainty in the nebular oxygen abundance scale.Comment: ApJS, in press; 52 emulateapj pages, 12 figures, and two appendices; v2: final abundances revised due to minor error; conclusions unchange

    The Impacts Of Bullying On The Main Character In The Movie “Laal Singh Chadha” (2022) By Advait Chandan

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    In this study, the researchers identified the types of bullying and described the impact of bullying on the main character of the film Laal Sings Chaddha. The data in this study are in the form of words and actions that contain elements of bullying in the film Laal Sings Chaddha. This research was conducted by analyzing data qualitatively. The research findings show that there are three effects of bullying on the main character of the film Laal Sings Chaddha: low psychological well-being, poor adjustment, and psychological stress. Low psychological well-being is the most dominant impact of bullying in Carrie's film. There is one type of bullying found in the film Laal Sings Chaddha: physical bullying. Physical bullying is the most dominant type of bullying found in the film Laal Sings Chaddha
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