2,852 research outputs found

    Recombinant DnaK orally administered protects axenic European sea bass against vibriosis

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    Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture and is a hindering factor for successful sustainable aquaculture of this commercially valuable species. Priming of the innate immune system through administration of immunostimulants has become an important approach to control disease outbreaks in marine fish larviculture. This study was conducted to evaluate immunostimulation by Escherichia coli HSP70 (DnaK) in axenic European sea bass larvae in order to protect the larvae against vibriosis. DnaK stimulates the immune response in crustaceans and juvenile fish against bacterial infections. The use of axenic fish larvae allows to study immunostimulation in the absence of an interfering microbial community. At 7 days post-hatching, larvae received a single dose of alginate encapsulated recombinant DnaK. Two non-treated control groups in which animals either received empty alginate microparticles (C1) or no alginante microparticles (C2 and C3) were included in the study. Eighteen hours later, all larvae, except the ones from group C3 (non-infected control) were challenged with V. anguillarum (10(5) CFU, bath infection). Mortality was daily recorded until 120 h post infection and at 18, 24, and 36 h post infection, larvae were sampled for expression of immune related genes. Results showed that V. anguillarum induced an immune response in axenic sea bass larvae but that the innate immune response was incapable to protect the larvae against deadly septicaemic disease. In addition, we showed that administration of alginate encapsulated DnaK to axenic European sea bass larvae at DAH7 resulted in a significant, DnaK dose dependent, upreglation of immune sensor, regulatory and effector genes. Significant upregulation of cxcr4, cas1 and especially of hep and dic was correlated with significant higher survival rates in V. anguillarum infected larvae. In the future recombinant DnaK might perhaps be used as a novel immunostimulant in sea bass larviculture

    Deep Multi-object Spectroscopy to Enhance Dark Energy Science from LSST

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    Community access to deep (i ~ 25), highly-multiplexed optical and near-infrared multi-object spectroscopy (MOS) on 8-40m telescopes would greatly improve measurements of cosmological parameters from LSST. The largest gain would come from improvements to LSST photometric redshifts, which are employed directly or indirectly for every major LSST cosmological probe; deep spectroscopic datasets will enable reduced uncertainties in the redshifts of individual objects via optimized training. Such spectroscopy will also determine the relationship of galaxy SEDs to their environments, key observables for studies of galaxy evolution. The resulting data will also constrain the impact of blending on photo-z's. Focused spectroscopic campaigns can also improve weak lensing cosmology by constraining the intrinsic alignments between the orientations of galaxies. Galaxy cluster studies can be enhanced by measuring motions of galaxies in and around clusters and by testing photo-z performance in regions of high density. Photometric redshift and intrinsic alignment studies are best-suited to instruments on large-aperture telescopes with wider fields of view (e.g., Subaru/PFS, MSE, or GMT/MANIFEST) but cluster investigations can be pursued with smaller-field instruments (e.g., Gemini/GMOS, Keck/DEIMOS, or TMT/WFOS), so deep MOS work can be distributed amongst a variety of telescopes. However, community access to large amounts of nights for surveys will still be needed to accomplish this work. In two companion white papers we present gains from shallower, wide-area MOS and from single-target imaging and spectroscopy.Comment: Science white paper submitted to the Astro2020 decadal survey. A table of time requirements is available at http://d-scholarship.pitt.edu/36036

    Coordinated Autonomic Managers for Energy Efficient Date Centers

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    The complexity of today’s data centers has led researchers to investigate ways in which autonomic methods can be used for data center management. Autonomic managers try to monitor and manage resources to ensure that the components they manage are self-configuring, self-optimizing, self-healing and self-protecting (so called “self-*” properties). In this research, we consider autonomic management systems for data centers with a particular focus on making data centers more energy-aware. In particular, we consider a policy based, multi-manager autonomic management systems for energy aware data centers. Our focus is on defining the foundations – the core concepts, entities, relationships and algorithms - for autonomic management systems capable of supporting a range of management configurations. Central to our approach is the notion of a “topology” of autonomic managers that when instantiated can support a range of different configurations of autonomic managers and communication among them. The notion of “policy” is broadened to enable some autonomic managers to have more direct control over the behavior of other managers through changes in policies. The ultimate goal is to create a management framework that would allow the data center administrator to a) define managed objects, their corresponding managers, management system topology, and policies to meet their operation needs and b) rely on the management system to maintain itself automatically. A data center simulator that computes its energy consumption (computing and cooling) at any given time is implemented to evaluate the impact of different management scenarios. The management system is evaluated with different management scenarios in our simulated data center

    Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro

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    This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophosphate at 3 h intervals from 0 to 12 h following maturation in culture or from 3 to 18 h following insemination. One-dimensional gel electrophoresis indicated that levels of protein phosphorylation are low prior to GVBD. However, the levels of protein phosphorylation at approximately 40 kDa, 27 kDa, 23 kDa and 18 kDa increased substantially following GVBD and then decreased gradually as maturation in culture progressed. In contrast, the levels of protein phosphorylation increased gradually in the oocytes following pronucleus formation. Further, two-dimensional gel electrophoresis indicated that the protein at approximately 18 kDa reversibly changed in the oocytes during maturation and fertilisation. These results indicate that the reversible changes of this phosphoprotein may be related to either cell cycle transition or pronucleus formation during maturation and fertilisation in bovine oocytes.</p

    A Neural TTS System with Parallel Prosody Transfer from Unseen Speakers

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    Modern neural TTS systems are capable of generating natural and expressive speech when provided with sufficient amounts of training data. Such systems can be equipped with prosody-control functionality, allowing for more direct shaping of the speech output at inference time. In some TTS applications, it may be desirable to have an option that guides the TTS system with an ad-hoc speech recording exemplar to impose an implicit fine-grained, user-preferred prosodic realization for certain input prompts. In this work we present a first-of-its-kind neural TTS system equipped with such functionality to transfer the prosody from a parallel text recording from an unseen speaker. We demonstrate that the proposed system can precisely transfer the speech prosody from novel speakers to various trained TTS voices with no quality degradation, while preserving the target TTS speakers' identity, as evaluated by a set of subjective listening experiments.Comment: Presented at Interspeech 202
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