18,595 research outputs found

    Half-Sandwich Rhodium Complexes with Releasable N-Donor Monodentate Ligands: Solution Chemical Properties and the Possibility for Acidosis Activation

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    Cancer chemotherapeutics usually have serious side effects. Targeting the special properties of cancer and activation of the anticancer drug in the tumor microenvironment in situ may decrease the intensity of the side effects and improve the efficacy of therapy. In this study, half-sandwich Rh complexes are introduced, which may be activated at the acidic, extracellular pH of the tumor tissue. The synthesis and aqueous stability of mixed-ligand complexes with a general formula of [Rh(η5-Cp*)(N,N/O)(N)]2+/+ are reported, where (N,N/O) indicates bidentate 8-quinolate, ethylenediamine and 1,10-phenanthroline and (N) represents the releasable monodentate ligand with a nitrogen donor atom. UV-visible spectrophotometry, 1H NMR, and pH-potentiometry were used to determine the protonation constants of the monodentate ligands, the proton dissociation constants of the coordinated water molecules in the aqua complexes, and the formation constants of the mixed-ligand complexes. The obtained data were compared to those of the analogous Ru(η6-p-cymene) complexes. The developed mixed-ligand complexes were tested in drug-sensitive and resistant colon cancer cell lines (Colo205 and Colo320, respectively) and in four bacterial strains (Gram-positive and Gram-negative, drug-sensitive, and resistant) at different pH values (5–8). The mixed-ligand complexes with 1-methylimidazole displayed sufficient stability at pH 7.4, and their activation was found in cancer cells with decreasing pH; moreover, the mixed-ligand complexes demonstrated antimicrobial activity in Gram-positive and Gram-negative bacteria, including the resistant MRSA strain. This study proved the viability of incorporating releasable monodentate ligands into mixed-ligand half-sandwich complexes, which is supported by the biological assays

    Conocimiento sobre fiebre y actitud de padres de familia en el tratamiento ante COVID-19, en Colegio Científico Albert Einstein, Lima- 2022

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    El presente estudio es determinar la relación que existe entre el nivel de conocimiento de fiebre y actitud de padres de familia ante COVID-19 en el Colegio Científico Albert Einstein, Lima-2022. Método. Tipo de investigación no experimental con un enfoque transeccional y correlacional simple. La población estuvo conformada por 190 padres de familia del Colegio Científico Albert Einstein, 2022. La muestra estuvo constituida por 128 padres de familia o apoderados, el muestreo fue no probabilístico por conveniencia, la técnica fue la encuesta y el instrumento basado en cuestionario en línea. Se realizó la validación del instrumento mediante Juicio de expertos y la confiabilidad por Alfa de Cronbach. Resultados: se observó que no guarda relación entre el nivel de conocimiento de fiebre y actitud de padres de familia ante COVID-19, obteniendo un coeficiente de correlación Spearman= -0,012, además hay una actitud favorable de los padres de familia a la detección de fiebre por COVID-19, Obteniendo un coeficiente de correlación Spearman =0,184*, seguido de un conocimiento y actitud negativo frente a la complicaciones febriles, obteniendo un coeficiente de correlación Spearman = 0,003, igual forma no guarda relación entre el nivel de conocimiento y actitud de padres de familia hacia el tratamiento de la fiebre por COVID-19, obteniendo un coeficiente de correlación Spearman = -0,075. Conclusiones: La investigación realizada concluye que no guarda una relación favorable entre el nivel de conocimientos sobre fiebre y actitudes de padres de familia respecto al COVID -19 en Colegio Científico Albert Einstein, pero hay actitud favorable de los padres de familia a la detección de fiebre por COVID-19.http://repositorio.unid.edu.pe/handle/unid/319?show=ful

    Living on the edge – An overview of invertebrates from groundwater habitats prone to extreme environmental conditions

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    Groundwater ecosystems from cold polar and circumpolar regions, hot springs, as well as those developed in salt, gypsum or in volcanic rocks are one of the environments considered to exhibit extreme environmental conditions such as low (below 0°C) or high (over 45°C) temperatures, hypersaline waters, or with elevated content of toxic gases like hydrogen sulfide or methane. They represent the “unseen ecosystem beneath our feet” and are inhabited by a large diversity of organisms, persisting and flourishing under severe environmental conditions that are usually hostile to the majority of organisms. These types of groundwater ecosystems are remarkable “evolutionary hotspots” that witnessed the adaptive radiation of morphologically and ecologically diverse species, whereas the organisms living here are good models to understand the evolutionary processes and historical factors involved in speciation and adaptation to severe environmental conditions. Here, we provide an overview of the groundwater invertebrates living in continental groundwater habitats prone to extreme environmental conditions in one or more physico-chemical parameters. Invertebrates are represented by a wide variety of taxonomic groups, however dominated by crustaceans that show specific adaptations mostly metabolic, physiologic, and behavioral. Symbiotic associations among bacteria and invertebrates are also discussed enlightening this biological interaction as a potential adaptation of different groundwater invertebrates to cope with severe environmental conditions. Given the high pressures that anthropogenic activities pose on groundwater habitats worldwide, we predict that several of these highly specialized organisms will be prone to extinction in the near future. Finally, we highlight the knowledge gaps and future research approaches in these particular groundwater ecosystems by using integrative-omic studies besides the molecular approach to shed light on genetic variation and phenotypic plasticity at species and populational levels.GRAPHICAL ABSTRAC

    Gehörte Geschichten

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    Auditive texts are on the rise: audio plays, readings, podcasts, audioguides, and electroacoustic experiments reveal the diversity of audible stories. The contributions in this volume systematize the genres of this diverse range of objects and model theoretical and methodological approaches that they apply in the analysis of media-specific examples. Semiotic approaches are accompanied by cultural studies perspectives

    Vino y Relaciones Internacionales: enodiplomacia

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    El artículo aborda el concepto enodiplomacia a partir del estudio y el análisis del potencial que tiene la industria del vino en su vertiente cultural y de relación humana internacional para que naciones, regiones y ciudades promuevan y desarrollen una imagen exterior asociada a ese universo. El vino como valor sustancial en las acciones de la diplomacia pública para interactuar con públicos extranjeros. Por lo tanto, este artículo se propone explorar el campo naciente de la enodiplomacia desde un punto de vista teórico-histórico, descriptivo y analítico que ayude a alcanzar el primer objetivo: aproximarse a una definición del término que delimite su alcance y trace sus ejes vertebradores. De ello deriva el segundo objetivo: por un lado, determinar su campo de acción (vino y relaciones internacionales) respecto a otras áreas concomitantes —la propia diplomacia oficial, la diplomacia comercial o la mercadotecnia internacional del vino encaminada a crear una marca país— con las que puede compartir líneas y técnicas; y por otro, distinguirla de la diplomacia cultural y de la gastrodiplomacia, áreas afines que también conviene deslindar. Una vez delimitado el campo conceptual, el tercer objetivo es analizar la acción diplomática en torno al vino desde su vertiente histórica: en el comercio, el arte y la cultura, es decir, la vinculación del vino a la civilización y a los aspectos de comunicación relacionados con la actividad diplomática. Tras recorrer la senda histórica, se investigan los actuales vectores de proyección del vino como embajador de la marca país y su acción diplomática (comunicación, relaciones exteriores); de ahí, a abordar el quinto: la actuación diplomática específica en torno al vino. Como último objetivo, es de esperar que del análisis y estudio de esta joven ciencia se descubran líneas de investigación que susciten interés científico, así como áreas de indagación que consoliden esta nueva disciplina. Así, el artículo se divide en cuatro grandes apartados. En el primero, se desarrollan los fundamentos teóricos que enmarcan la investigación. En los siguientes se abre la discusión sobre el nuevo concepto; se estudia la vinculación del vino a la civilización y a los aspectos de comunicación relacionados con la actividad diplomática y su proyección en dichas acciones. Finalmente, se extraen conclusiones y se proponen líneas de actuación en consonancia con los objetivos planteados

    Whole-genome sequencing and phylogenetic analysis capture the emergence of a multi-drug resistant Salmonella enterica serovar Infantis clone from diagnostic animal samples in the United States

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    IntroductionSalmonella enterica is a major cause of foodborne illness in the United States. A multi-drug resistant (MDR) emergent Salmonella Infantis (ESI) with a megaplasmid (pESI) was first identified in Israel and Italy and subsequently reported worldwide. The ESI clone carrying an extended spectrum β-lactamase blaCTX-M-65 on a pESI-like plasmid and a mutation in the gyrA gene has recently been found in the United States in poultry meat.MethodsWe analyzed the phenotypic and genotypic antimicrobial resistance, genomics and phylogeny of 200 S. infantis isolates from animal diagnostic samples.ResultsOf these, 33.5% were resistant to at least one antimicrobial and 19.5% were multi-drug resistant (MDR). Eleven isolates from different animal sources were phenotypically and genetically similar to the ESI clone. These isolates had a D87Y mutation in the gyrA gene conferring reduced susceptibility to ciprofloxacin and harbored a combination of 6–10 resistance genes: blaCTX-M-65, aac(3)-IVa, aadA1, aph(4)-Ia, aph(3′)-Ia, floR, sul1, dfrA14, tetA, and fosA. These 11 isolates carried class I and class II integrons and three virulence genes: sinH, involved in adhesion and invasion, ybtQ and ybtP, associated with iron transport. These isolates were also closely related to each other (separated by 7 to 27 SNPs) and phylogenetically related to the ESI clone recently found in the U.S.DiscussionThis dataset captured the emergence of the MDR ESI clone in multiple animal species and the first report of a pESI-like plasmid in isolates from horses in the U.S

    The most common dermatological manifestations of inflammatory bowel diseases

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    Inflammatory bowel diseases are increasingly common problem in clinical practice. This condition is caused by both the growing morbidity and more accurate and widely available diagnostic methods, affecting the rising recognition.  It is worth remembering, that clinical presentation of inflammatory bowel disease is not limited to gastrointestinal tract.  Dermatological symptoms are an important part in the diagnosis of inflammatory bowel diseases, because they can occur as the first manifestation of the beginning of the disease process, as well as be a prognostic factor for the occurrence of exacerbations of the disease. This is an invaluable source of information on the patient's clinical condition, affecting the effectiveness of his treatment in the early stages of the disease, and allowing more efficient control of its course.  This has an enormous impact on the prognosis and comfort of patients' lives. In the following article, available medical studies on extraintestinal skin manifestations, clinical presentation and treatment options have been reviewed

    Towards a Burning Method: how might the contemporary performer build on the legacy of Grotowski's Total Act?

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    Towards a Burning Method is a practical-theoretical investigation in the context of the contemporary theatre practice of Jerzy Grotowski's ideas, particularly the Total Act from the Theatre of Productions period. The study employs a practice of confrontation with Grotowski's ideas rather than identification, and text and practice are inseparable, interpenetrating each other. The method of confrontation (which is one of Grotowski's ideas) means, in the context of my research, a dialogue which through its dynamics creates a performative situation, stimulates the deepening of knowledge, and seeks its own answers. The resulting performance Burning Method - Four Lectures on Conditional Love is also integral to the theoretical reflections, both representing and containing Grotowski's ideas and my response to them. Other practical activities include video notes of process, classes with students and workshops. Oneof the important results of the research is to broaden the understanding of what performance is, what kind of forms it takes in relation to the Total Act. The thesis consists of four chapters. Chapter One introduces the cultural-historical context of the emergence of the Total Act, while the following chapters are a guide to understanding the Act in practice. The final chapter is entirely devoted to my practical activities through a dialogue on contemporary performance, its lineage of origin and projective reflections for the future. A reference point or dialogue partner on contemporary theatre is mainly Grotowski's book Towards a Poor Theatre but also Artaud's The Theatre and its Double. The research has helped me understand what performance work might result from thecontinuation of a distinctly masculine Polish Romantic tradition, and what new possibilities emerge from my perspective as a female performer and theatre-maker. In reading Grotowski (and confronting it in practice) I discover the potential for creative freedom, with the rigour of attention to the execution of ideas. At the same time, working with video projections I verify my thinking about the performer's body and space by finding another dimension of expression in the image in relation to the audience. I also believe that actor training from this tradition has no temporal, cultural or other limitations as its essence is the search for the living impulse in the performer's body. Summing up the research is an opening for further explorations revealing the potential and currency of Grotowski's ideas for today
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