Development of freeze-thaw processing technique for disaggregation of indurated mudrocks and enhanced recovery of calcareous microfossils

Microfossil extraction from indurated mudrocks is widely acknowledged as challenging, especially for foraminifera. Here we report development of the freeze–thaw extraction method through the addition of rapid heating, detergent and ultrasound stages. We use indurated mudrock samples from the Toarcian (Early Jurassic) of Yorkshire, UK to assess the effectiveness and develop the freeze–thaw method. We compare our results from freeze–thaw with those from standard foraminifera processing techniques, including the use of hydrogen peroxide. Processing by freeze–thaw increased the degree of mudrock disaggregation and resulted in no damage or dissolution of foraminifera. Following the freeze–thaw method with treatment in white spirit and sodium hexametaphosphate aided the separation of foraminifera from the disaggregated clays and was twice as efficient as pressure washing. Samples processed with hydrogen peroxide contained damaged microfossils and an under representation of delicate calcareous foraminifera. Many other studies of indurated mudrocks have used hydrogen peroxide to extract foraminifera, and this might have resulted in apparently barren intervals. The freeze–thaw method outlined here provides a low-cost, low-risk and successful method of disaggregating and extracting calcareous microfossils from indurated mudrocks. We anticipate our method may be relevant for other fossil groups and merits further development

Significant Miocene larger foraminifera from South Central Java

The Gunung Sewu area of South Central Java, Indonesia during Mid Miocene, Langhian-Serravallian (Tf1-Tf2), was deposited in a large area of warm, very shallow-marine water. Coralline algae and abundant larger benthic foraminifera dominate the carbonate lithologies. Larger benthic foraminifera from previously unstudied sections in South Central Java are described and figured. They have led to an understanding of sequence stratigraphic and facies relationship of Miocene carbonates in Indonesia. Thirteen larger foraminifera species are described and illustrated. A detailed biostratigraphical studies of The phylogeny Katacycloclypeus annulatus - K. martini and the gradual evolution from Austrotrillina asmariensis into A. howchini are recognised. Analysis of the larger benthic foraminifera has allowed accurate dating of the carbonate sections studied using the East Indian Letter Classification

The study of the umbilical system in planktonic foraminifera in relation with depth of the Ziarat-kola section at the Maastrichtian, Central Alborz, IRAN

The main aim of this research is study of the planktonic foraminifera morphogroups distinction genus to perform by changing the umbilicus area in Ziarat-kola section to find novel results. Therefore, original objection at this research considers the cause of organizing umbilicus structures (Lip, Portici, Tegilla) at this protests. it seems that phylogeny trend entirely the changes of planktonic foraminifera changing from lip at primary morphogroup to tegilla at development shape which continued this phylogeny trend opening become entirely umbilicus that this trend accompanied to increasing deep. Therefore, the study of planktonic foraminifera morphotype, and recognizing, the obtained results from planktonic foraminifera analysis percent and their comparison with umbilicus structures area diagrams at this section indicats the increasing morphotype three accompany with increase in sea level that here dominated portici and tegilla structure with compressed opening and with decrease of morphotype three which showed decrease sea level, opening structure (lip) dominated. These trends follows the from Pascal law at Ziarat-kola section of the research

Paleoecology of the Maastrichtian and Danian sediment at North of Central Alborz, Iran, based on Planktonic foraminifera

In order to study the fossil contents of the Ziarat-kola section for biostratigraphical purposes the 212 meters was sampled at Central Alborz. The sequence is mainly made up of monotonous grey- green to light grey marl. Based on Planktonic foraminifera age of Maastrichtian-Danian is determined to the section.

Four zones were determined by the benthic foraminifera morphogroup for showing oxygen and productivity change. The approximate low oxygen and high food supply has been indicates zone of one that increase infauna to epifauna morphotype percent is low (nearness 60% infouna, 40% epifauna). In zone two, with due attention to equal percent of Epifauna ratio to infauna, intermediate oxygen and food supply is anticipated. The decrasing epifauna foraminifera and increasing infauna demonstrates low oxygen and high food supply condition at zone of three. In zone four, increasing epifauna (approximate 90 percent) that are indicated high oxygen condition and low food supply. Analysis of planktonic foraminifera assemblage at this section with Cretaceous biostratigraphical provinces is indicating a close similarity with Tethyan provinces

Benthic foraminifera show some resilience to ocean acidification in the northern Gulf of California, Mexico.

The version on PEARL: Corrected proofs are Articles in Press that contain the authors' corrections. Final citation details, e.g., volume/issue number, publication year and page numbers, still need to be added and the text might change before final publication. Although corrected proofs do not have all bibliographic details available yet, they can already be cited using the year of online publication and the DOI , as follows: author(s), article title, journal (year), DOIExtensive CO2 vents have been discovered in the Wagner Basin, northern Gulf of California, where they create large areas with lowered seawater pH. Such areas are suitable for investigations of long-term biological effects of ocean acidification and effects of CO2 leakage from subsea carbon capture storage. Here, we show responses of benthic foraminifera to seawater pH gradients at 74-207m water depth. Living (rose Bengal stained) benthic foraminifera included Nonionella basispinata, Epistominella bradyana and Bulimina marginata. Studies on foraminifera at CO2 vents in the Mediterranean and off Papua New Guinea have shown dramatic long-term effects of acidified seawater. We found living calcareous benthic foraminifera in low pH conditions in the northern Gulf of California, although there was an impoverished species assemblage and evidence of post-mortem test dissolution

The new and reinstated genera of agglutinated foraminifera published between 1986 and 1996

In the 10 years following the publication of "Foraminiferal Genera and their Classification" by Loeblich & Tappan (1987), some 91 new genera of agglutinated foraminifera have been proposed by various authors. Additionally, at least four of the genera listed by Loeblich & Tappan as junior synonyms have been resurrected by subsequent authors. This compilation is an attempt at bringing together the nomenclatorial changes to the agglutinated foraminiferal genera that have appeared in the accessible literature

The niche of benthic foraminifera, critical thresholds and proxies

Ecological studies of benthic foraminifera are carried out to explain patterns of distribution and the dynamics of communities. They are also used to provide data to establish proxy relationships with selected factors. According to niche theory, the patterns of distribution of benthic foraminifera are controlled by those environmental factors that have reached their critical thresholds. For each species, in variable environments, different factors may be limiting distributions both temporally and spatially. For a species or an assemblage to be useful as a proxy its abundance must show a strong correlation with the chosen factor. Since numerous factors influence each species, it is only in those environments where the majority of factors show little variation but one particular factor shows significant variation that the proxy relationship for that factor can be determined. On theoretical grounds, the reliability of using foraminiferal abundance as a proxy of a selected environmental factor should be restricted to the range close to the upper and lower thresholds. For oxygen, foraminifera are potential proxies for the lower limits but once oxygen levels rise to values of perhaps >1 or 2 ml l-1, there is no longer a relationship between oxygen levels and abundance. By contrast, the flux of organic matter over a large range shows a sufficiently close relationship with foraminiferal assemblages so that transfer functions can be derived for the deep sea. However, the relationship at species level is far less clear cut. Much more accurate estimates of primary productivity and modern organic flux rates are required to improve the determination of past flux rates

Miocene deep water agglutinated foraminifera from Viosca Knoll, offshore Louisiana (Gulf of Mexico)

An exploration well from the Gulf of Mexico, Amoco Viosca Knoll-915, has been studied in order to document the Neogene foraminiferal assemblages. Ditch cuttings samples from the Amoco V.K. 915 well yielded diverse assemblages of agglutinated and calcareous benthic foraminifera over a stratigraphic interval of 2940 m. Three species associations can be identified in the studied interval; the stratigraphical location of these associations is evident when total agglutinated species abundance for each sampling interval is plotted. In this study we use a combination of morphotype habitat preference and test functional morphology to interpret depositional environments. The associations indicate a change from a well-ventilated water column, to the development of a strong oxygen minimum zone characterised by alveolar foraminifera. The species composition of the lowermost association indicates a depositional environment dominated by fine-grained overbank fines and channel levee deposits, in agreement with sedimentological data. Colour plates of key agglutinates species are presented, created using digital image manipulation techniques (Palaeovision Technique, NHM)

Coupled Mg/Ca and clumped isotope analyses of foraminifera provide consistent water temperatures

The reliable determination of past seawater temperature is fundamental to paleoclimate studies. We test the robustness of two paleotemperature proxies by combining Mg/Ca and clumped isotopes (Δ47) on the same specimens of core top planktonic foraminifera. The strength of this approach is that Mg/Ca and Δ47 are measured on the same specimens of foraminifera, thereby providing two independent estimates of temperature. This replication constitutes a rigorous test of individual methods with the advantage that the same approach can be applied to fossil specimens. Aliquots for Mg/Ca and clumped analyses are treated in the same manner following a modified cleaning procedure of foraminifera for trace element and isotopic analyses. We analysed eight species of planktonic foraminifera from coretop samples over a wide range of temperatures from 2 to 29°C. We provide a new clumped isotope temperature calibrations using subaqueous cave carbonates, which is consistent with recent studies. Tandem Mg/Ca–Δ47 results follow an exponential curve as predicted by temperature calibration equations. Observed deviations from the predicted Mg/Ca-Δ47 relationship are attributed to the effects of Fe-Mn oxide coatings, contamination, or dissolution of foraminiferal tests. This coupled approach provides a high degree of confidence in temperature estimates when Mg/Ca and Δ47 yield concordant results, and can be used to infer the past δ18O of seawater (δ18Osw) for paleoclimate studies

Modern seawater acidification: The response of foraminifera to high-CO<inf>2</inf> conditions in the Mediterranean Sea

The seas around the island of Ischia (Italy) have a lowered pH as a result of volcanic gas vents that emit carbon dioxide from the sea floor at ambient seawater temperatures. These areas of acidified seawater provide natural laboratories in which to study the long-term biological response to rising CO2 levels. Benthic foraminifera (single-celled protists) are particularly interesting as they have short life histories, are environmentally sensitive and have an excellent fossil record. Here, we examine changes in foraminiferal assemblages along pH gradients at CO2 vents on the coast of Ischia and show that the foraminiferal distribution, diversity and nature of the fauna change markedly in the living assemblages as pH decreases. © 2010 Geological Society of London