Study on single calibration of near infrared reflectance spectroscopy for lignin

The study of single calibration of near infrared reflectance srectroscory for lignin was done using 50 feed samples and its paired 50 fecal samrles from dairy cows. These samrles were collected from digestion trials. in which the in vivo digestibility values were deternlined, The dlgC-ostibillty of these feed samples were separated into two grours, namely Italian ryegruss only (n= 19) and combinution of Italian ryegrass and concentmte (n=31), The NIRS srectm or these samrles were recorded using I'aciflc Scientific (Neotec) model 6500 (Perstorp Analytical, Silver Srring, MD) instrument equirrl'Cl with lSI software (InfraSoft International, Port Matilda, PAl for analysIs, Three arproach methods for devcloring the single calibmtion were, (I) Lignin in feed and feces was detcn;1ined usmg lignin calibratIOn developed from Italian ryegrass (L1RG): (2) Lignin of ft.'Cd and feces was detennined lIsing lignin calibmtion developed ITom feces (LFEC): and (3) Lignin oC feed and feces were detemlined using the caiibmtion equation for lignin develorcd from Si.U11rles of fecdstufl's wld feces (LMIX). The resulb showed that lignin of feed and feces in il~ function for digestibility marker could be detennincd by single calibration developed from samrles consisting of Italian ryegm~s, concentrates and feces

Management of Child Feces: Current Disposal Practices

This research brief examines how children's feces are disposed of, a neglected area of research, policy, and program intervention, and there is very little evidence of effective strategies for increasing the safe disposal of children's feces

PENGARUH RASIO FESES SAPI DENGAN TANDAN KOSONG KELAPA SAWIT TERHADAP KADAR FOSFOR DAN KADAR KALIUM KOMPOS

This study aims to determine the effect of the ratio of cow feces to TKKS on phosphorus levels and potassium levels of compost. The research method used in this study is an experimental method with a completely randomized design (CRD). In this composting process, the treatments were P1 (60% cow feces + 40% TKKS) P2 (50% cow feces + 50% TKKS) P3 (40% cow feces + 60% TKKS).The method used for the measurement of compost content uses the spectrophotometric method (Uv-Vis). Based on the results showed that from all ratios of cow feces to oil palm empty fruit bunches had no significant effect on potassium content and phosphorus content of compost. Potassium content produced = P1 0.28%, P2 0.27%, P3 0.31%. This value has met the standard requirements of SNI 19-7030-2004 potassium levels for compost which is 0.20%. Phosphorus content of the resulting compost = P1 0.23%, P2 0.22%, P3 0.23%. This value has met the standard requirements of SNI 19-7030-2004 for compost phosphorus, which is >0.10%. The nature of the compost produced in this study has also met the compost standard. The results of this study can be applied to soil or plants

Integrating Multiple Analytical Datasets to Compare Metabolite Profiles of Mouse Colonic-Cecal Contents and Feces.

The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed

FECES STANDARD MONEY: BEYOND TRANSACTIONS

Department of Urban and Environmental Engineering (Convergence of Science and Arts)Feces Standard Money (fSM), is a complementary currency that is different from other currencies in a number of ways. It is the first currency to adopt feces as its standard. In a world where objects and people are thought of as "goods and services," reality is compressed into conceptions of "use value" or "utility???. However, in the fSM system, feces and food waste that have traditionally and culturally been classified as ???human waste??? are used to produce biogas, creating value. Feces then becomes a representation of a new conception of value - one based on abundance instead of scarcity. This study aims to explore how the use of fSM can facilitate a redefinition of sustainable wealth. It begins by exploring neoclassical and modern theories of money and their relationship to the current state of money. It argues that economics??? failure to adequately account for the role of money as a basis of social relations contributes to the current unsustainable economic system. Building on the background and philosophical underpinnings of fSM, it postulates that money based on a feces standard might be a possible solution to developing a monetary system that can serve as the basis of social relations and facilitation of exchange as a means of instigating social change in attitudes towards global challenges like inequality and climate change. Social network analysis is used to investigate the social footprint of fSM in a game simulation of the fSM system. It is found that the mechanisms of fSM has the potential to imbue the network with tight knit connections -knots- that can contribute to a more inclusive monetary system.clos

Vertebrate DNA in Fecal Samples from Bonobos and Gorillas: Evidence for Meat Consumption or Artefact?

Background: Deciphering the behavioral repertoire of great apes is a challenge for several reasons. First, due to their elusive behavior in dense forest environments, great ape populations are often difficult to observe. Second, members of the genus Pan are known to display a great variety in their behavioral repertoire; thus, observations from one population are not necessarily representative for other populations. For example, bonobos (Pan paniscus) are generally believed to consume almost no vertebrate prey. However, recent observations show that at least some bonobo populations may consume vertebrate prey more commonly than previously believed. We investigated the extent of their meat consumption using PCR amplification of vertebrate mitochondrial DNA (mtDNA) segments from DNA extracted from bonobo feces. As a control we also attempted PCR amplifications from gorilla feces, a species assumed to be strictly herbivorous. Principal Findings: We found evidence for consumption of a variety of mammalian species in about 16% of the samples investigated. Moreover, 40% of the positive DNA amplifications originated from arboreal monkeys. However, we also found duiker and monkey mtDNA in the gorilla feces, albeit in somewhat lower percentages. Notably, the DNA sequences isolated from the two ape species fit best to the species living in the respective regions. This result suggests that the sequences are of regional origin and do not represent laboratory contaminants. Conclusions: Our results allow at least three possible and mutually not exclusive conclusions. First, all results may represent contamination of the feces by vertebrate DNA from the local environment. Thus, studies investigating a species' diet from feces DNA may be unreliable due to the low copy number of DNA originating from diet items. Second, there is some inherent difference between the bonobo and gorilla feces, with only the later ones being contaminated. Third, similar to bonobos, for which the consumption of monkeys has only recently been documented, the gorilla population investigated (for which very little observational data are as yet available) may occasionally consume small vertebrates. Although the last explanation is speculative, it should not be discarded a-priori given that observational studies continue to unravel new behaviors in great ape species

Uptake and fecal excretion of Coxiella burnetii by Ixodes ricinus and Dermacentor marginatus ticks

Background: The bacterium Coxiella burnetii is the etiological agent of Q fever and is mainly transmitted via inhalation of infectious aerosols. DNA of C. burnetii is frequently detected in ticks, but the role of ticks as vectors in the epidemiology of this agent is still controversial. In this study, Ixodes ricinus and Dermacentor marginatus adults as well as I. ricinus nymphs were fed on blood spiked with C. burnetii in order to study the fate of the bacterium within putative tick vectors. Methods: Blood-feeding experiments were performed in vitro in silicone-membrane based feeding units. The uptake, fecal excretion and transstadial transmission of C. burnetii was examined by quantitative real-time PCR as well as cultivation of feces and crushed tick filtrates in L-929 mouse fibroblast cells and cell-free culture medium. Results: Ticks successfully fed in the feeding system with engorgement rates ranging from 29% (D. marginatus) to 64% (I. ricinus adults). Coxiella burnetii DNA was detected in the feces of both tick species during and after feeding on blood containing 105 or 106 genomic equivalents per ml blood (GE/ml), but not when fed on blood containing only 104 GE/ml. Isolation and cultivation demonstrated the infectivity of C. burnetii in shed feces. In 25% of the I. ricinus nymphs feeding on inoculated blood, a transstadial transmission to the adult stage was detected. Females that molted from nymphs fed on inoculated blood excreted C. burnetii of up to 106 genomic equivalents per mg of feces. Conclusions: These findings show that transstadial transmission of C. burnetii occurs in I. ricinus and confirm that I. ricinus is a potential vector for Q fever. Transmission from both tick species might occur by inhalation of feces containing high amounts of viable C. burnetii rather than via tick bites

Fecal non-aureus Staphylococci are a potential cause of bovine intramammary infection

The presence of non-aureus staphylococci (NAS) in bovine rectal feces has recently been described. Similar to other mastitis causing pathogens, shedding of NAS in the environment could result in intramammary infection. The objective of this study was to investigate whether NAS strains present in feces can cause intramammary infection, likely via teat apex colonization. During a cross-sectional study in 5 dairy herds, samples were collected from the habitats quarter milk, teat apices, and rectal feces from 25%, 10%, and 25% of the lactating cows, respectively, with a cow serving as the source of one type of sample only. Samples from clinical mastitis cases were continuously collected during the 1-year study period as well. The 6 most prevalent NAS species, Staphylococcus (S.) chromogenes, S. cohnii, S. devriesei, S. equorum, S. haemolyticus, and S. hominis, were further subtyped by random amplification of polymorphic deoxyribonucleic acid polymerase chain reaction (RAPD-PCR), when the same NAS species was present in the same herd in the three habitats. For S. chromogenes, S. cohnii, S. devriesei, and S. haemolyticus, the same RAPD type was found in rectal feces, teat apices, and quarter milk, indicating that fecal NAS can infect the mammary gland. For S. hominis and S. equorum, we were unable to confirm the presence of the same RAPD types in the three habitats

Relation between serology of meat juice and bacteriology of tonsils and feces for the detection of enteropathogenic Yersinia spp. in pigs at slaughter

The association between positive serology and culture detection of Yersinia spp. in individual pigs was determined. Pieces of diaphragm from 370 pig carcasses were collected for serological analysis, and tonsils and feces of the same carcass were collected for bacteriological analysis. Detection of anti-Yersinia antibodies in meat juice samples was done using an indirect enzyme-linked immunosorbent assay (ELISA) based on Yops (Yersinia outer proteins). Tonsils and feces were tested for the presence of enteropathogenic Yersinia spp. by direct plating on cefsulodin–irgasan–novobiocin agar plates. Of the 370 meat juice samples, 241 (65.1%) gave a positive serological reaction using a cutoff value of 20%. Enteropathogenic Yersinia spp. (Yersinia enterocolitica serotype O:3 and Yersinia pseudotuberculosis) were found in tonsils of 161 pigs and feces of 30 pigs. Recovery of enteropathogenic Yersinia from the tonsils was highly correlated with positive serotiters, whereas no correlation was found between serology and fecal excretion. Results demonstrated that serology has an acceptable sensitivity, but a relatively low specificity for the rapid detection of enteropathogenic Yersinia spp. in tonsils of pigs at slaughter