2 research outputs found
Proinflammatory Caspase A Activation and an Antiviral State Are Induced by a Zebrafish Perforin after Possible Cellular and Functional Diversification from a Myeloid Ancestor
14 pages, 9 figuresIn mammals, perforins play a central role in the granule-dependent
cell death induced by natural killer T cells and cytotoxic
T lymphocytes, and participate both in the defense
against virus-infected and neoplastic cells and in the recognition
of nonself molecules by the immune system. Little is
known about fish perforin genes. We examined the zebrafish
with the aim of increasing our knowledge about the role of
perforins. We characterized 6 perforin genes in the zebrafish
genome, and we studied them at the evolutionary level in
combination with expression patterns in several tissues and
cell populations, during both larval development and in the
course of a viral infection. Our results suggest the specialization
of different cell types in the production of perforins.
Moreover, functional diversification during the evolution of
these molecules could be inferred from this study. In particular,
one of the genes, prf19b , which is mainly produced by
myeloid cells, seemed to be involved in antiviral defense,
conferring protection after an in vivo infectionThis work was funded by the projects CSD2007–00002
‘Aquagenomics’ and AGL2014–51773-C3 from the Spanish Ministerio
de EconomÃa y Competitividad. M. Varela received a predoctoral
grant from the JAE Program (funded by the CSIC and
European Social Funds).Peer reviewe