X-ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats

Background: Non-invasive methods to study gastrointestinal (GI) motility are of high interest, particularly in chronic studies. Amongst these, radiographic techniques after contrast intragastric administration may offer many advantages. In previous studies, we have successfully and reproducibly applied these techniques together with a semiquantitative analysis method to characterize the effect of different drugs, acutely or repeatedly administered in rat models, but we have never before used these techniques in mice. These are very convenient in basic research. Our aim was to determine if our method is also valid in mice. Additionally, we determined the effect of morphine on GI motor function in both species. Methods: Animals received an intraperitoneal administration of morphine (at 10 and 5 mg/kg for rats and mice, respectively). Twenty min later, barium contrast (at 2 g/mL) was gavaged (2.5 and 0.4 mL for rats and mice respectively) and serial X-rays were obtained 0-8 h after contrast. X-rays were analyzed as previously described, using a semiquantitative score to build motility curves for each GI region. Key Results: Motility was much faster in mice than in rats for all GI regions. Morphine at the doses used significantly depressed motility in both species to a similar extent if the whole gut or the upper GI regions (stomach, small intestine) were considered, although its effect seemed to be more intense in the lower GI regions (caecum, colorectum) in rats than in mice. Conclusions & Inferences: We have validated our X-rays method for its use in mice.This work was supported by Ministerio de Educacion y Ciencia (SAF2012-40075-C02-01) and Comunidad de Madrid (S2010/BMD- 2308Peer Reviewe