The obtention of simian virus 40 recombinants carrying d(CG.GC)n, d(CA.GT)n and d(CT.GA)n sequences. Stability of the inserted simple repeating sequences

4 pages, 3 figures.-- PMID: 2820728 [PubMed].-- Available online Mar 3, 2005.A general strategy for the introduction of simple repeating DNA sequences into the simian virus 40 (SV40) has been developed. SV40 recombinants carrying d(CG · GC)5, d(CA · GT)30 or d(CT · GA)22 insertions at either the TaqI site (position 4739) or the HpaII site (position 346) were obtained and the stability of the inserted DNA sequences studied. The palindromic potentially Z-DNA-forming d(CG · GC)n sequence was found to be highly unstable when compared to either d(CA · GT)n or d(CT · GA)n.This work was supported by grants from the Comision Asesora de lnvestigacion Cientifica y Tecnica, the Consejo Superior de Investigaciones Cientzficas (CSIC) and the Comissio Interdepertamental de Recerca i Innovacio Tecnologica. J. M. C. was a recipient of a doctoral fellowship from the Ministerio de Educacion y Ciencia. M. J. E. was supported by a fellowship from the Medical Research Council of Canada. A. R. C. was a postdoctoral fellow from the C. S. I. C.Peer reviewe