3 research outputs found
Correlation of molecular markers and biological properties in Verticillium dahliae and the possible origins of some isolates
9 pages; 3 tables; 1 figureHaploid and amphihaploid Verticillium dahliae
isolates were studied using PCR-based molecular markers which: (i)
discriminate the defoliating and nondefoliating pathotypes (two primer pairs INTD2f/ r and INTND2f/ r), and (ii) are speciesspecific
(primer pair 19/22). The results were compared with some known biological and other molecular properties of
the isolates. Five discrete sequences of the 19/22 amplicon were found. Sequence 4 was associated with both defoliating
isolates from Spain and nondefoliating isolates from Spain and USA; these pathotypes were separated by the primer pairs
INTD2f/ r and INTND2f/ r, but the data showed that the primer espdef01 (derived from the 19/22 amplicon) cannot be
used for this purpose. Amplicon sizes and sequences with primers 19/22 divided amphihaploid isolates from crucifers
(thought to be interspecific hybrids) into those corresponding to the previously reported
α
and
β
groups. The
β
-group
isolates had either sequence 4 or 5 (these two differing by a single base). The distinct amplicon sequence 3 given by the
α
-group isolates demonstrated that the
V. dahliae
-like ‘parent’ of this group was molecularly unlike any haploid isolate
yet studied. The overall results are discussed in relation to phytosanitary considerations and the probability of defoliating
or crucifer pathotypes arising
de novo
within Europe, either by selection or by interspecific hybridizations.European Commission (Framework Programme 5;
project reference number QLRT-1999-1523)Peer reviewe
Correlation of molecular markers and biological properties in Verticillium dahliae and the possible origins of some isolates
9 pages; 3 tables; 1 figureHaploid and amphihaploid Verticillium dahliae
isolates were studied using PCR-based molecular markers which: (i)
discriminate the defoliating and nondefoliating pathotypes (two primer pairs INTD2f/ r and INTND2f/ r), and (ii) are speciesspecific
(primer pair 19/22). The results were compared with some known biological and other molecular properties of
the isolates. Five discrete sequences of the 19/22 amplicon were found. Sequence 4 was associated with both defoliating
isolates from Spain and nondefoliating isolates from Spain and USA; these pathotypes were separated by the primer pairs
INTD2f/ r and INTND2f/ r, but the data showed that the primer espdef01 (derived from the 19/22 amplicon) cannot be
used for this purpose. Amplicon sizes and sequences with primers 19/22 divided amphihaploid isolates from crucifers
(thought to be interspecific hybrids) into those corresponding to the previously reported
α
and
β
groups. The
β
-group
isolates had either sequence 4 or 5 (these two differing by a single base). The distinct amplicon sequence 3 given by the
α
-group isolates demonstrated that the
V. dahliae
-like ‘parent’ of this group was molecularly unlike any haploid isolate
yet studied. The overall results are discussed in relation to phytosanitary considerations and the probability of defoliating
or crucifer pathotypes arising
de novo
within Europe, either by selection or by interspecific hybridizations.European Commission (Framework Programme 5;
project reference number QLRT-1999-1523)Peer reviewe