The Schizosaccharomyces pombe endo-1,3-β-glucanase Eng1 contains a novel carbohydrate binding module required for septum localization

13 pages, 6 figures.-- et al.Cell separation in Schizosaccharomyces pombe is achieved through the concerted action of the Eng1 endo-β-1,3-glucanase and the Agn1 endo-α-1,3-glucanase, which are transported to the septum and localize to a ring-like structure that surrounds the septum. Correct localization of these hydrolases requires the presence of both the septins and the exocyst. In this work, we show that the glucanase Eng1 contains a region at the C-terminus that acts as a carbohydrate-binding module (CBM) and that it is not present in other members of glycoside hydrolases family 81 (GH81). In vitro, the purified CBM has affinity for β-1,3-glucan chains with a minimum degree of polymerization of 30 glucose units. Deletion of the CBM results in a protein that is largely defective in complementing the separation defect of eng1Δ mutants. This defect is due to a reduction in the catalytic activity against insoluble substrates and to a defect in targeting of Eng1 to the septum, as the truncated protein localizes to the lateral cell wall of the cell. Thus, the targeting of Eng1 to the primary septum requires not only trans-factors (septins and the exocyst complex) but also a cis-element localized to the C-terminus of the protein.This research was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (BFU2004-00778 and BFU2007-60390/BMC) to C.V.-A. and F.R. and from the European Community (LSHB-CT-2004–511952) to C.V.-A. and J.P.L. A.B. Martín-Cuadrado, J. Encinar del Dedo and M. de Medina-Redondo are recipients of fellowships from the Ministerio de Ciencia y Tecnología (Spain).Peer reviewe