2 research outputs found
Effects of light intensity and addition of carotene rich Dunaliella salina live cells on growth and antioxidant activity of Solea senegalensis Kaup (1858) larval and metamorphic stages
14 pages, 4 figuresSenegal sole Solea senegalensis larval and metamorphic stages were exposed to a range of light
intensities (200, 1000 and 2000 lx) in cultures with or without supplementation of b-carotenerich
live Dunaliella salina cells. Antioxidant biomarkers such as superoxide dismutase (SOD),
catalase (KAT), total glutathione peroxidase (t-GPX) and malondialdehyde (MDA) were
determined in larval and metamorphic stages. Growth was not affected (P > 0 05) either by light
intensity or D. salina supplementation. Survival after metamorphosis was also unaffected by D.
salina supplementation (mean S.E. 81 0 2 5% against 80 6 2 9% those fed the control
algal diet) or light intensity (mean S.E. 74 3 4 9% for 200 lx, 85 1 2 7% for 1000 lx and 82 8
5 2% for 2000 lx, respectively). Light intensity affected (P < 0 05) KAT and t-GPX throughout
development. SOD was only affected in metamorphosing larvae. The highest KAT and t-GPX
activities were detected when the lowest light intensity (200 lx) was used. Light had no effect
(P > 0 05) on MDA at any stage. Supplementing the diet with D. salina did not affect SOD,
KAT or t-GPX and there was no interaction (P > 0 05) with light intensity. MDA was the only
biomarker whose activity was significantly (P < 0 05) reduced when D. salina was supplemented
to the larval rearing tanks. The effect of D. salina supplementation was only detected in
metamorphosing larvae, whose MDA levels were noticeably higher than in earlier stages. These
results are evidence of the antiperoxidative effect of b-carotene from live algae in the larval
rearing process of marine fishes.This research has been co-financed by the European Regional Development Fund.
INTERREG IIIB, project 091–AAAG, and by the Spanish Ministry for Science and
Technology (MCYT project AGL2003-03558).Peer reviewe