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    Changes on enzymatic activity and on sarcoplasmic and myofibrillar proteins of frozen‐stored hake (Merluccius merluccius) pre‐treated by high pressure

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    8 pages, 2 figures, 3 tablesHigh‐pressure (HP) pre‐treatments were applied on European hake (Merluccius merluccius) followed by a frozen accelerated experiment (−10 °C). A central composite design ranging pressure levels (150–450 MPa) and frozen storage time (0–150 days) was used, being evaluated the enzymatic activities and muscle proteins. Acid phosphatase and calpain activities decreased after 150 days of frozen storage (58%/56% and 38%/56% for non‐/HP‐treated samples, respectively). Cathepsin B showed higher reductions (98%) for longer storage times. Furthermore, HP and frozen storage did not affect significantly cathepsin D activity, only slightly decreasing at 169 MPa. Furthermore, HP seemed to not affect myofibrillar proteins, while sarcoplasmic proteins were clearly affected by HP and frozen storage time, resulting in a reduction of about 53% or 23% for 431 or 450 MPa, respectively. Thus, HP could be used to lowering the deleterious effect of proteases on frozen European hakeThanks are due to FCT/MEC for the ïŹnancial support to the QOPNA research Unit (FCT UID/QUI/00062/2013) as well as the PhD. grants of Liliana G. Fidalgo (SFRH/BD/96984/2013), through national funds and where applicable co-ïŹnanced by the FEDER, within the PT2020 Partnership Agreement. The ïŹnancial support provided by Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia (ES) is also acknowledged (Project # ED431B 2016/009) and of CSIC-Spain through the Research Project 2017-70E032.Peer reviewe
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