2 research outputs found
Changes on enzymatic activity and on sarcoplasmic and myofibrillar proteins of frozenâstored hake (Merluccius merluccius) preâtreated by high pressure
8 pages, 2 figures, 3 tablesHighâpressure (HP) preâtreatments were applied on European hake (Merluccius merluccius) followed by a frozen accelerated experiment (â10 °C). A central composite design ranging pressure levels (150â450 MPa) and frozen storage time (0â150 days) was used, being evaluated the enzymatic activities and muscle proteins. Acid phosphatase and calpain activities decreased after 150 days of frozen storage (58%/56% and 38%/56% for nonâ/HPâtreated samples, respectively). Cathepsin B showed higher reductions (98%) for longer storage times. Furthermore, HP and frozen storage did not affect significantly cathepsin D activity, only slightly decreasing at 169 MPa. Furthermore, HP seemed to not affect myofibrillar proteins, while sarcoplasmic proteins were clearly affected by HP and frozen storage time, resulting in a reduction of about 53% or 23% for 431 or 450 MPa, respectively. Thus, HP could be used to lowering the deleterious effect of proteases on frozen European hakeThanks are due to FCT/MEC for the ïŹnancial support to the QOPNA research Unit (FCT UID/QUI/00062/2013) as well as the PhD. grants of Liliana G. Fidalgo (SFRH/BD/96984/2013), through national funds and where applicable co-ïŹnanced by the
FEDER, within the PT2020 Partnership Agreement. The ïŹnancial support provided by Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia (ES) is also acknowledged (Project # ED431B 2016/009) and of CSIC-Spain through the Research Project 2017-70E032.Peer reviewe