Gas Chromatographic Method for Analysis of Conjugated Linoleic Acids Isomers (c9t11, t10c12, and t9t11) in Broth Media as Application in Probiotic Studies

A gas chromatography (GC) procedure is assayed for analysis of conjugated linoleic acid (CLA) isomers cis-9, trans-11-octadecadienoic (c9t11); trans-10, 12 cis-octadecadienoic (t10c12); and trans-9, trans-11-octadecadienoic (t9t11) in culture broth by GC using NaOH-BF3 in methanol for methylating and a long capillary (100 m) high-polarity column. Repeatability of the method is assessed; the coefficient of variation for CIA isomers ranges from 4.62 for c9t11 to 8.19 for t9t11. Recovery ranges between 88.01 and 89.76, with a mean value of 89.06 for all CLA isomers studied. This method may be considered advantageous for analysis of CLA isomers in probiotics cultures samples.L. Alonso thanks the Ministery of Education of Spain for the support during his time in the Food and Agricultural Product Research and Technology Center at Oklahoma State University. This project was supported under Project H-2293 and Sitlington Endowment Funds.Peer Reviewe