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Characterization of Collagen from Different Discarded Fish Species of the West Coast of the Iberian Peninsula
13 páginas, 5 tablas, 3 figuras.-- This is an open Access article. Non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly attributed, cited, and is not altered, transformed, or built upon in any way, is permitted. The moral rights of the named author(s) have been asserted.Skin collagen of six discarded fish species was analyzed and compared. Acid
soluble collagen (ASC) was extracted; a characteristic sodium dodecyl sulfate-
polyacrylamide gel electrophoresis (SDS-PAGE) profile for type I collagen
was obtained, except for Chimaera mostrosa. Contents of collagen
calculated from HPro (31.85% average) were higher than those determined
from ASC extracts (17.75% average), with Galeus spp. being the species with
the higher percentage. Amino acid analysis revealed the typical composition
of collagen, with very few differences among species. Specific profiles
were obtained after protease digestion. Denaturation temperature of ASC
correlated well with imino and hydroxyproline contents.
Results demonstrate the feasibility of using the obtained collagens in
different industrial applicationsThis work was funded by European Union FEDER POCTEP Project 0330_IBEROMARE_1_P, European Union FEDER
Atlantic Area Project MARMED-2011-1/164, Xunta de Galicia Project 10TAL011E, and a contract with the “Cooperativa
de Armadores del Puerto de Vigo” and FROM (Ministery of Environment, Rural and Maritime Affairs, Spain)Peer reviewe