Conserved gene regulation during acute inflammation between zebrafish and mammals

9 páginas, 4 figuras, 1 tabla.-- This work is licensed under a Creative Commons Attribution 4.0 International LicenseZebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases. However, in a context where even mice have been questioned as a valid model for these analysis, a systematic study evaluating the reproducibility of human and mammalian inflammatory diseases in zebrafish is still lacking. In this report, we characterize the transcriptomic regulation to lipopolysaccharide in adult zebrafish kidney, liver, and muscle tissues using microarrays and demonstrate how the zebrafish genomic responses can effectively reproduce the mammalian inflammatory process induced by acute endotoxin stress. We provide evidence that immune signaling pathways and single gene expression is well conserved throughout evolution and that the zebrafish and mammal acute genomic responses after lipopolysaccharide stimulation are highly correlated despite the differential susceptibility between species to that compound. Therefore, we formally confirm that zebrafish inflammatory models are suited to study the basic mechanisms of inflammation in human inflammatory diseases, with great translational impact potentialThis work was funded by the projects CSD2007–00002 “Aquagenomics” and AGL2014-51773-C3 from the Spanish Ministerio de Economía y Competitividad, and 201230E057 “Proyecto Intramural Especial, PIE”, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC). P. Pereiro and M. Varela received predoctoral grants from the Ministerio de Educación (F.P.U. fellowship AP2010-2408) and the JAE Program (funded though the CSIC and European Social Funds), respectivelyPeer reviewe