2 research outputs found
Protein changes in stored ultra-high-temperature-treated milks studied by capillary electrophoresis and high-performance liquid chromatography
IDF Symposium on Heat Treatment and Alternative Methods, SEP 06-08, 1995The evolution of direct and indirect ultra-high-temperature-treated milk during storage is studied.
Capillary electrophoresis and HPLC analysis of whey is performed. Both techniques allow the
quantitation of R-lactalbumin and â-lactoglobulin, providing comparable results at the beginning
of the storage and making it possible to estimate the thermal treatment to which milk has been
subjected. Besides, the capillary zone electrophoresis method allows differentiation between the
proteolysis products caused by plasmin and by some proteases from psychrotrophic bacteria.
Isoelectric focusing of the casein fraction allows identification of the degradation products originating
from both types of proteases.We thank Dr. C. San José for kindly providing the P.
fluorescens B52 protease. Ms. C. Talavera is thanked
for excellent technical assistance.Peer reviewe