Hydrolyzed caseinomacropeptide conjugated galactooligosaccharides support the growth and enhance the bile tolerance in lactobacillus strains

In this study bioactive caseinomacropeptide was conjugated with prebiotic galactooligosaccharides (hCMP:GOS) by Maillard reaction to synthesize value added prebiotic compounds to Lactobacillus strains. Growth study showed the ability of hCMP:GOS to serve as a sole carbon source for Lactobacillus strains. A significant amount of acetate and lactate was detected in cell free culture supernatant by HPLC. It demonstrated the ability of Lactobacillus strains to ferment the hCMP:GOS as a carbon source. In addition, hCMP:GOS grown Lactobacillus cells exhibited enhanced bile tolerance and retained 90% viability. Overall results of this study indicate that the hCMP conjugated GOS can be potential multipurpose prebiotic substrates to enhance the growth and bile tolerance in Lactobacillus strains and serve as a fermentable substrate to produce beneficial metabolites in the host. © 2012 American Chemical Society.We gratefully acknowledge financial support from Junta de Castilla-La Mancha and European regional development fund (ERDF) (POII10-0178-4685), from Comunidad Autónoma de Madrid (ALIBIRD S2009/AGR-1469), and from Ministerio de Ciencia e Innovación (AGL2011-27884). S.C.R. received support from a USDA Food Safety Consortium and a USDA-NIFSI (2008-51110-04339) grant. O.H.-H. thanks the CSIC for a JAE-PreDoc PhD grant.Peer Reviewe