Live imaging in Drosophila: The optical and genetic toolkits

Biological imaging based on light microscopy comes at the core of the methods that let us understanding morphology and its dynamics in synergy to the spatiotemporal distribution of cellular and molecular activities as the organism develops and becomes functional. Non-linear optical tools and superesolution methodologies are under constant development and their applications to live imaging of whole organisms keep improving as we speak. Genetically coded biosensors, multicolor clonal methods and optogenetics in different organisms and, in particular, in Drosophila follow equivalent paths. We anticipate a brilliant future for live imaging providing the roots for the holistic understanding, rather than for individual parts, of development and function at the whole-organism level. © 2014 Elsevier Inc.The Consolidated Groups Program of the Generalitat de Catalunya and DGI and Consolider Grants from the Ministry of Economy and Competitivity of Spain to E.M.B. and an Explora Grant to E.R. supported this workPeer Reviewe