Cell adhesion on surface patterns generated by the photocrosslinking of hyperbranched polyesters with a trisdiazonium salt

Aliphatic hyperbranched polyesters of different generation, having different nucleophilic groups (such as hydroxyl or sulfonate) in the periphery, have been photocrosslinked by using a trifunctional low molecular weight diazonium salt. The photocrosslinking process, that does not require photoinitiator, allows to prepare polymeric materials that have shown not to cause cytotoxicity when used as substrate for cell growth. Furthermore, cell adhesion studies carried out in polymeric micropatterns generated in glass by direct laser writing, show that cells preferentially proliferate on the structure demonstrating cell affinity for the material.This work was supported by the Spanish MINECO projects MAT2011-27978-C02-01, MAT2011-27978-C02-02 and MAT2011-26851-C02-01, ARAID project for young scientists, ERC-Starting Grant NANOPUZZLE and FEDER funding (EU). M. Lomba thanks the Gobierno de Aragón (Spain) for a PhD. Grant. JM de la Fuente thanks ARAID for financial support.Peer Reviewe