Synthesis and modification of polyurethane for immobilization of Thermomyces lanuginosus (TLL) lipase for ethanolysis of fish oil in solvent free system

We report the synthesis of polyurethane (PU) with polyethylene glycol (PEG) by miniemulsion polymerization as technique to produce a support for enzyme immobilization and stabilization. In order to study the influence of molar weight and concentration of PEG in the enzyme-support interaction, different PEG (400, 4000 and 6000 Da) was used during the synthesis of PU-PEG support to immobilize Thermomyces lanuginosus lipase (TLL). The enzymatic derivatives were effectively used in the production of polyunsaturated fatty acids (PUFAs), particularly derivative TLL-PU-PEG6000. This derivative showed the best results in terms of Km (0.183 mM) and Vmax (45.79 mmol/min/mL), calculated by Lineweaver-Burk plot (double reciprocal plot) and catalytic efficient (250.22), 100% of yield immobilization and recovered activity, reaching the highest values to the ethyl esters production (260 mM ethyl esters. U-1) making this derivative a potential low cost catalyst for ethanolysis reactions in solvent free systemThe authors thank the financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the Programa de Doutorado Sanduíche no Exterior, Project BEX 2569/14-0; the direction of the Instituto de Investigación en Ciencias de la Alimentación, CIAL-CSIC for helping in the development of this research work, and Laboratório Central de Microscopia Eletrônica (LCME) at Federal University of Santa Catarina for the microscopy analyzes.Peer Reviewe