3 research outputs found
Extravascular hemolysis and complement consumption in Paroxysmal Nocturnal Hemoglobinuria patients undergoing eculizumab treatment
Los datos asociados con este artículo están disponibles en: http://dx.doi.org/10.1016/j.imbio.2016.09.002.Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hemolytic anemia characterized by complement-mediated intravascular hemolysis that is effectively treated with eculizumab. However, treatment responses are reported heterogeneous with some patients presenting residual hemolysis and requiring RBC transfusions. Recent reports have shown that both extravascular hemolysis and incomplete C5 blockade can explain these suboptimal hematological responses. Here we have tested our eculizumab-treated PNH patients (n = 12) for signs of hemolysis and assessed complement biomarkers. Patients were also genotyped for complement receptor 1 (CR1, CD35) and C5 polymorphisms and evaluated for free eculizumab in plasma. We report that 10 patients (83%) present parameters suggesting persistent hemolysis, although they did not require additional transfusions. Seven of them (58%) become direct Coombs-test positive as a consequence of treatment, including all patients carrying the low-expression CR1-L allele. CH50 and sC5b-9 assays demonstrate that the persistent low-level hemolysis identified in our treated patients is not a consequence of incomplete C5 blockade, supporting that this hemolysis, as has been suggested previously, results from the extravascular removal of C3 opsonized PNH erythrocytes. We also show that continuous alternative pathway activation in eculizumab-treated individuals carrying the CR1-L allele results in abnormally decreased levels of C3 in plasma that could, potentially, increase their susceptibility to bacterial infections. Finally, we encourage a routine evaluation of free eculizumab levels and terminal pathway activity to personalize eculizumab administrationIn this study S.R de C. is supported by the Spanish “Ministerio de Economía y Competitividad” (SAF2011-26583) and the Autonomous Region of Madrid (S2010/BMD-2316
Hemolysis in eculizumab-treated PNH patients
35 p.-3 fig.-4 tab. Subías, Marta et al.Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hemolytic anemia
characterized by complement-mediated intravascular hemolysis that is effectively
treated with eculizumab. However, treatment responses are reported heterogeneous
with some patients presenting residual hemolysis and requiring RBC transfusions.
Recent reports have shown that both extravascular hemolysis and incomplete C5
blockade can explain these suboptimal hematological responses. Here we have tested
our eculizumab-treated PNH patients (n=12) for signs of hemolysis and assessed
complement biomarkers. Patients were also genotyped for complement receptor 1
(CR1, CD35) and C5 polymorphisms and evaluated for free eculizumab in plasma. We
report that 10 patients (83%) present parameters suggesting persistent hemolysis,
although they did not require additional transfusions. Seven of them (58%) become
direct Coombs-test positive as a consequence of treatment, including all patients
carrying the low-expression CR1-L allele. CH50 and sC5b-9 assays demonstrate that
the persistent low-level hemolysis identified in our treated patients is not a
consequence of incomplete C5 blockade, supporting that this hemolysis, as has been
suggested previously, results from the extravascular removal of C3 opsonized PNH
erythrocytes. We also show that continuous alternative pathway activation in
eculizumab-treated individuals carrying the CR1-L allele results in abnormally
decreased levels of C3 in plasma that could, potentially, increase their susceptibility to
bacterial infections. Finally, we encourage a routine evaluation of free eculizumab
levels and terminal pathway activity to personalize eculizumab administration.S.RdeC is supported by the Spanish “Ministerio de Economía y Competitividad” (SAF2011-26583) and the Autonomous Region of Madrid (S2010/BMD-2316).Peer reviewe