1 research outputs found
Multi locus sequence typing of oenological Saccharomyces cerevisiae strains
6 p谩ginas -- 2 TablasThis study describes the application of a multilocus sequence typing (MLST) analysis for molecular
discrimination at the strain level of Spanish wine yeast strains. The discrimination power of MLST is
compared to mitochondrial RFLP analysis. Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes
were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. Ten polymorphic
sites were found in the five loci analyzed showing 13 different genotypes, with 11 of them
represented by only one strain. RFLP analysis of the same 18 wine yeast strains showed seventeen
different mitochondrial patterns. Phylogenetic relationships among the strains analyzed, inferred by
MLST data, showed wine isolates of S. cerevisiae as a rather homogeneous group. The discrimination
potential of mitochondrial RFLP analysis was superior to the MLST scheme used in this work. However,
MLST analysis allowed an easy construction of reliable phylogenetic trees. MLST analysis offers the
possibility of typing wine S. cerevisiae strains simultaneously to the study of the genetic relationship
among themThis work was supported by
the Spanish Ministerio de Educaci贸n y Ciencia (Consolider
CSD2007-00063); and the Comunidad de Madrid local Government
(grant S-505/AGR-0153).Peer reviewe