2 research outputs found
Adhesion and detachment kinetics of several strains of Staphylococcus aureus subsp. aureus under three different experimental conditions
7 páginas, 5 figuras, 4 tablasThe kinetics of adhesion of five Staphylococcus aureus subsp. aureus strains (CECT 976, 4459, 4465, 4466 and 5191) to polypropylene
at 25 1C in the absence of nutrients (PBS medium) were initially compared. Those strains with the highest (CECT 4459) and the lowest
(CECT 976) adhesion levels were selected for further studying the effects of a nutrient-rich adhesion-promoting medium (TSB plus 1%
glucose—TSBG) as well as of a conditioning film consisting of dried mussel cooking juices (MCJ) on adhesion to and detachment from
polypropylene surfaces. Adhesion kinetics were properly described by an empirical model in the absence of conditioning film. The
maximum adhesion level was much higher in the presence of TSBG than in PBS, decreasing sharply in both cases after 10–15 h. In
contrast, adhesion increased exponentially during 25 h in the presence of dried MCJ. Clear differences were thus found in different media,
and it suggests that cleaning strategies should vary under different conditions. The comparison of the adhesion strengths under the
different experimental conditions showed that the persistence was highest when biofilms were formed on MCJ, which indicates that cells
would remain longer as a source of cross-contamination. Some biofilms were examined by electronic microscopy, and different structures
were observed under the different experimental conditions. It is concluded that the study of biofilm formation by S. aureus is necessary to
establish efficient control systems in the food industry.Peer reviewe
Adhesion and detachment kinetics of several strains of Staphylococcus aureus subsp. aureus under three different experimental conditions
7 páginas, 5 figuras, 4 tablasThe kinetics of adhesion of five Staphylococcus aureus subsp. aureus strains (CECT 976, 4459, 4465, 4466 and 5191) to polypropylene
at 25 1C in the absence of nutrients (PBS medium) were initially compared. Those strains with the highest (CECT 4459) and the lowest
(CECT 976) adhesion levels were selected for further studying the effects of a nutrient-rich adhesion-promoting medium (TSB plus 1%
glucose—TSBG) as well as of a conditioning film consisting of dried mussel cooking juices (MCJ) on adhesion to and detachment from
polypropylene surfaces. Adhesion kinetics were properly described by an empirical model in the absence of conditioning film. The
maximum adhesion level was much higher in the presence of TSBG than in PBS, decreasing sharply in both cases after 10–15 h. In
contrast, adhesion increased exponentially during 25 h in the presence of dried MCJ. Clear differences were thus found in different media,
and it suggests that cleaning strategies should vary under different conditions. The comparison of the adhesion strengths under the
different experimental conditions showed that the persistence was highest when biofilms were formed on MCJ, which indicates that cells
would remain longer as a source of cross-contamination. Some biofilms were examined by electronic microscopy, and different structures
were observed under the different experimental conditions. It is concluded that the study of biofilm formation by S. aureus is necessary to
establish efficient control systems in the food industry.Peer reviewe