Nuclear versus cytosolic activity of the yeast Hog1 MAP kinase in response to osmotic and tunicamycin-induced ER stress

© 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. We examined the physiological significance of the nuclear versus cytosolic localization of the MAPK Hog1p in the ability of yeast cells to cope with osmotic and ER (endoplasmic reticulum) stress. Our results indicate that nuclear import of Hog1p is not critical for osmoadaptation. Plasma membrane-anchored Hog1p is still able to induce increased expression of GPD1 and glycerol accumulation. This is a key osmoregulatory event, although a small production of the osmolyte coupled with the nuclear import of Hog1p is sufficient to provide osmoresistance. On the contrary, the nuclear activity of Hog1p is dispensable for ER stress adaptation.This research was supported by the Comisión Interministerial de Ciencia y Tecnología project (AGL2010-17516) from the Ministry of Economy and Competitiveness of Spain (MINECO). SG-M was supported by a pre-doctoral fellowship within the “JAE” Program (CSIC).Peer Reviewe