Triphenyltin and tributyltin, single and in combination, promote imposex in the gastropod Bolinus brandaris

8 pages, 2 tablesSpecimens of Bolinus brandaris (neogastropod) were injected with a single dose of 500 ng/g body weight (b.w.) of tributyltin chloride (TBTCl) or triphenyltin chloride (TPTCl), or a mixture of both compounds (250 ng TBT/g b.w.+250 ng TPT/g b.w.), for a period of up to 31 days. At the end of 4 weeks, significant increases in the female penis size of those gastropods injected with TBT (Po0:05), TPT (Po0:05), or the mixture TBT+TPT (Po0:01) were recorded. In parallel, a group of animals was injected with the neuropeptide APGWamide but this compound failed to promote imposex, suggesting that APGWamide is not involved in imposex promotion in B. brandaris. Acetylcholinesterase activity, a biomarker of neurotoxicity, was determined in the neuroganglia at the end of the experiment, but no significant differences among treatments were found. Overall, these results support the hypothesis that TPT also acts as an endocrine disrupter in this neogastropod species. Our observations also highlight, for the first time, synergistic effects of organotin mixtures having imposex promotion as an endpoint.This work was partly possible through a visit grant to M.M. Santos from ‘‘Junta de Andalucía.’’ M.M. Santos also acknowledged financial support from the project ‘‘Portuguese Foundation for Science and Technology PDCTM/MAR/15284/99.’’Peer reviewe