1 research outputs found
Involvement of pore-forming molecules in immune defense and development of the Mediterranean mussel (Mytilus galloprovincialis)
15 p谩ginas, 9 figuras, 3 tablasThe membrane attack complex and perforin (MACPF) superfamily is one of the largest families of poreforming
molecules. Although MACPF proteins are able to destruct invading microbes, several MACPF
proteins play roles in embryonic development, neural migration or tumor suppression. We describe two
apextrin-like proteins (ApelB and ApelP) and one MACPF-domain-containing protein (Macp) in Mytilus
galloprovincialis. The two apextrin-like proteins did not present any conserved domain. The Macp protein
contained the membrane/attack complex domain and its signature motif. Gene expression during larval
development was analyzed by RT-PCR. There was a stage-specific up-regulation of the three proteins,
suggesting that they play a role in development. Apextrin-like proteins were highly expressed at blastula
and trochophore stage, whereas Macp was expressed at veliger stage. RT-PCR revealed up-regulation
of the three genes in tissues and hemocytes from adults treated with bacteria and pathogen-associated
molecular patterns, suggesting that they may be involved in the immune response.This work has been funded by the EU Projects IMAQUANIM (CT-
2005-007103), REPROSEED (245119) and by the National project
AGL2008-05111/ACU from the Spanish Ministerio de Ciencia e
Innovaci贸n.Peer reviewe