Effect of ivermectin on the liver of gilthead seabream Sparus aurata: a proteomic approach

23 p., 2 tablas, 4 figuras y bibliografíaGilthead sea bream Sparus aurata is the most commercialized Mediterranean aquacultured fish species. Ivermectin has recently(experimentally) started to be used to control ectoparasitic infestations in Mediterranean cultured marine fish. The potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35g) following oral administration at the recommended dose of 0.2mg kg-1 fish for 10d. Difference Gel Electrophoresis Technology (DIGE) was used to study the effect of this treatment in gilthead sea bream liver protein profile under routine culture conditions. The 2D-DIGE protein maps obtained were analyzed using the DeCyder 6.5 software. The results obtained showed significant changes in the expression of 36 proteins respect to the control group. Among these proteins, six increased in abundance, and 30 decreased. Spot showing differential expression respect to the control were analyzed by mass spectrometry and database search, which resulted in three positive identifications corresponding to hepatic proteins involved in lipid metabolism (apoA-I), oxidative stress responses and energy generation (beta-globin, ATP synthase subunit beta). These proteins have not been previously associated to invermectin effect.This work was funded by projects GV06B-351 “Generalitat Valenciana” and CTM 2006-14279-CO2-01/MAR MEC-FEDER. This research was performed within the framework of a concerted action between Spain and Greece (HG-2004-0016). I. Varó was a recipient of a “Ramón y Cajal” contract at the University of Valencia from the “Ministerio de Educación y Ciencia (Spain)”.Peer reviewe

Effect of ivermectin on the liver of gilthead seabream Sparus aurata: a proteomic approach

23 p., 2 tablas, 4 figuras y bibliografíaGilthead sea bream Sparus aurata is the most commercialized Mediterranean aquacultured fish species. Ivermectin has recently(experimentally) started to be used to control ectoparasitic infestations in Mediterranean cultured marine fish. The potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35g) following oral administration at the recommended dose of 0.2mg kg-1 fish for 10d. Difference Gel Electrophoresis Technology (DIGE) was used to study the effect of this treatment in gilthead sea bream liver protein profile under routine culture conditions. The 2D-DIGE protein maps obtained were analyzed using the DeCyder 6.5 software. The results obtained showed significant changes in the expression of 36 proteins respect to the control group. Among these proteins, six increased in abundance, and 30 decreased. Spot showing differential expression respect to the control were analyzed by mass spectrometry and database search, which resulted in three positive identifications corresponding to hepatic proteins involved in lipid metabolism (apoA-I), oxidative stress responses and energy generation (beta-globin, ATP synthase subunit beta). These proteins have not been previously associated to invermectin effect.This work was funded by projects GV06B-351 “Generalitat Valenciana” and CTM 2006-14279-CO2-01/MAR MEC-FEDER. This research was performed within the framework of a concerted action between Spain and Greece (HG-2004-0016). I. Varó was a recipient of a “Ramón y Cajal” contract at the University of Valencia from the “Ministerio de Educación y Ciencia (Spain)”.Peer reviewe